FUNCTIONAL VALIDATION OF USP-LIKE GENE CLONED FROM INDIAN MULBERRY (Morus alba L.)
| dc.contributor.advisor | NATARAJA KARABA, N. | |
| dc.contributor.author | JEEVAN, A. R. | |
| dc.date.accessioned | 2020-10-07T06:19:39Z | |
| dc.date.available | 2020-10-07T06:19:39Z | |
| dc.date.issued | 2019-11-02 | |
| dc.description.abstract | Universal stress proteins (USPs) are a group of proteins, whose expression is stimulated by a wide range of stresses. Ubiquitously present in wide set of genomes ranging from archae to plants and animals, the biological and biochemical functions of these proteins are not completely uncovered. The present study examines the relevance of a USP like gene (MaUSP-like), previously identified and cloned from Indian mulberry, in imparting stress tolerance in model system tobacco (Nicotiana tabacum). In-silico expression analysis performed using the Arabidopsis homologue of MaUSP-like, and invivo analysis in mulberry indicated that the gene was responsive to diverse abiotic stresses. Experiments conducted using transgenic seeds (T1 generation) showed no significant impact on normal growth and development in tobacco. However, under salt stress, the transgenic seedlings exhibited better germination, seedling vigor with better lateral roots and total chlorophyll content than the non transgenic plants. Under osmotic stress (D-mannitol), the transgenic seeds had higher germination percentage, and exhibited better survival under heat stress. Transgenic plants showed better phenotype and recovery after drought, with significantly higher relative water content, cell membrane stability, root biomass and less water loss rate. There was delay in leaf senescence under salinity and drought stress in transgenic lines and transgenic cell lines sustained cell division under salt stress, in-vitro. The transgenic lines were found to be heat stable compared to non transgenic plants. The study demonstrates the role of MaUSP-like identified from Indian mulberry in improving cell tolerance and imparting abiotic stress tolerance in tobacco. | en_US |
| dc.identifier.other | Th-12522 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810152711 | |
| dc.keywords | Significant, Tobacco, Transgenic plants. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 125 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU | en_US |
| dc.sub | Crop Physiology | en_US |
| dc.theme | FUNCTIONAL VALIDATION OF USP-LIKE GENE CLONED | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | FUNCTIONAL VALIDATION OF USP-LIKE GENE CLONED FROM INDIAN MULBERRY (Morus alba L.) | en_US |
| dc.type | Thesis | en_US |