Effect of salinity on morphophysiological characteristics of Dalbergia sissoo Roxb.

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dc.contributor.advisorVarshney, U.K.
dc.contributor.authorNisha
dc.date.accessioned2016-10-18T10:47:34Z
dc.date.available2016-10-18T10:47:34Z
dc.date.issued2015
dc.description.abstractThe objectives of present investigation were to observe the effect of chloride-dominated salinity on morpho-physiological characteristics of Dalbergia sissoo saplings and to study the protein profile by SDS-PAGE in control versus saline treated leaf. Two experiments were conducted in the screen house on one year old Dalbergia sissoo saplings subjected to nutrient supplemented chloride dominated salinity treatments at 4 EC level viz., 0 (control), 4, 8, and 12dSm-1. The first experiment was conducted with an objective to study the effect of chloride-dominated salinity on some morphophysiological characteristics of Dalbergia sissoo saplings. The study revealed the deleterious effects of salinity on plant height, stem, leaf and root dry mass, root length and root volume. All these parameters suffered a significant reduction with progressive increase of EC level in the growth medium. Chlorophyll content, starch and total leaf protein, RWC significantly reduced while accumulation of total soluble solids, total soluble sugar and proline in leaves was noticed with the rise of salinity in the growth medium. Membrane injury index of leaves drastically increased at 8 and 12dSm-1 EC levels as compared to the control due to membrane injury caused by toxic level of ions. Significant accumulation Na+, Cl- and SO4 2- and decline of K+ contents in leaves was quiet evident. In the second experiment protein profile by SDS-PAGE in control vs. saline treated plant leaves at the termination of experiment was studied. The study showed variation in the protein profile of leaves at different EC levels. Under 4dSm-1 EC level of salinity 3 new protein bands of molecular weight 12.5, 24.0 and 47.8 kDa were synthesized de novo and one protein band of 23.7 kDa was disappeared while under 8dSm-1 EC level, two new protein bands of molecular weight 16.3 and 17.0 kDa were de novo synthesis and one protein band 18.4 was disappeared. At 12dSm-1 EC level of salinity 2 new protein bands 21.2 and 28.1 kDa molecular weight were synthesized de novo while one protein band of molecular weight 28.6 disappeared. Salinity in general was found deleterious to Dalbergia sissoo saplings as there was a retarding effect on almost all the morphological, physiological and biochemical parameters studied. The saplings although survived at the highest level of salinity studied (12dSm-1) by osmotic adjustment and synthesis of salt induced proteins but an overall diminished growth indicated the salt sensitivity of the tree species.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/80815
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.research.problemEffect of salinity on morphophysiological characteristics of Dalbergia sissoo Roxb.en_US
dc.subBotanyen_US
dc.subjectNitrogen, Genotypes, Developmental stages, Diseases, Sorghum, Yields, Topping, Drying, Grain, Areaen_US
dc.themeEffect of salinity on morphophysiological characteristics of Dalbergia sissoo Roxb.en_US
dc.these.typeM.Sc
dc.titleEffect of salinity on morphophysiological characteristics of Dalbergia sissoo Roxb.en_US
dc.typeThesisen_US
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