Effect of salinity on morphophysiological characteristics of Dalbergia sissoo Roxb.
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Date
2015
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Publisher
CCSHAU
Abstract
The objectives of present investigation were to observe the effect of chloride-dominated
salinity on morpho-physiological characteristics of Dalbergia sissoo saplings and to study the protein
profile by SDS-PAGE in control versus saline treated leaf. Two experiments were conducted in the
screen house on one year old Dalbergia sissoo saplings subjected to nutrient supplemented chloride
dominated salinity treatments at 4 EC level viz., 0 (control), 4, 8, and 12dSm-1. The first experiment
was conducted with an objective to study the effect of chloride-dominated salinity on some
morphophysiological characteristics of Dalbergia sissoo saplings. The study revealed the deleterious
effects of salinity on plant height, stem, leaf and root dry mass, root length and root volume. All these
parameters suffered a significant reduction with progressive increase of EC level in the growth
medium. Chlorophyll content, starch and total leaf protein, RWC significantly reduced while
accumulation of total soluble solids, total soluble sugar and proline in leaves was noticed with the rise
of salinity in the growth medium. Membrane injury index of leaves drastically increased at 8 and
12dSm-1 EC levels as compared to the control due to membrane injury caused by toxic level of ions.
Significant accumulation Na+, Cl- and SO4
2- and decline of K+ contents in leaves was quiet evident. In
the second experiment protein profile by SDS-PAGE in control vs. saline treated plant leaves at the
termination of experiment was studied. The study showed variation in the protein profile of leaves at
different EC levels. Under 4dSm-1 EC level of salinity 3 new protein bands of molecular weight 12.5,
24.0 and 47.8 kDa were synthesized de novo and one protein band of 23.7 kDa was disappeared while
under 8dSm-1 EC level, two new protein bands of molecular weight 16.3 and 17.0 kDa were de novo
synthesis and one protein band 18.4 was disappeared. At 12dSm-1 EC level of salinity 2 new protein
bands 21.2 and 28.1 kDa molecular weight were synthesized de novo while one protein band of
molecular weight 28.6 disappeared. Salinity in general was found deleterious to Dalbergia sissoo
saplings as there was a retarding effect on almost all the morphological, physiological and biochemical
parameters studied. The saplings although survived at the highest level of salinity studied (12dSm-1) by
osmotic adjustment and synthesis of salt induced proteins but an overall diminished growth indicated
the salt sensitivity of the tree species.
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Keywords
Nitrogen, Genotypes, Developmental stages, Diseases, Sorghum, Yields, Topping, Drying, Grain, Area