Rapid diagnostic methods for detection of bovine tuberculosis in resource poor settings
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Date
2021
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Abstract
A study was conducted in 30 numbers of Jersey cross cattle at Post Graduate Research Institute in
Animal Sciences, Tamil Nadu Veterinary and Animal Sciences University for rapid detection of
Mycobacteriosis. Lymph node smear and sputum swabs were collected from the study population and
were subjected to Auramine -O-Fluorescent staining and Loop mediated isothermal Amplification
(LAMP). Out of 30 samples, 16 (53.33%) samples were found as positive by both test methods with 22
samples (73.33%) sparing positive only by Auramine O Fluorescent staining. There were only 6 (20%)
samples which showed negative to Auramine O Fluorescent staining but positive to LAMP method. It
was concluded that these two diagnostic methods may be considered as parallel tests for rapid diagnosis
of Mycobacteriosis where early diagnostic methods like Interferon Gamma assay are not feasible for
isolation and quarantine of positive animals before confirmation by gold standard culture method.
Description
TNV_TPI_2021_10(12)_946-948
Keywords
Veterinary Science, Veterinary Public Health and Epidemiology