MOLECULAR GENETIC CHARACTERIZATION OF ONGOLE AND DEONI CATTLE
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Date
2003-03
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
The investigation was undertaken to study the genetic variability within and between Ongole and Deoni breeds of cattle using microsatellite markers. Ten microsatellite markers viz. ARO23, ARO62, ARO85, BMS1716, BMS2057, BMS2270, BMS2840, BMS2847, BtDIAS1 and PZE46 were used for this purpose. Eight markers were found to be polymorphic. Dinucleotides were observed to be more polymorphic than trinucleotides. The overall mean number of alleles per locus was found to be 3.8 in Ongole and 3.5 in Deoni breed. Statistical analysis of the data was done to calculate allele frequency, observed and expected heterozygosity and polymorphism information content. Few alleles were observed to be breed specific with 3 to 24 % frequency. Some other alleles were found to be present at higher frequency in one breed than in the other breed. The overall expected heterozygosity of Ongole breed was 0.46 and it was 0.50 for Deoni breed, where as the overall observed heterozygosity was 0.30 and 0.41 for Ongole and Deoni, respectively, while the polymorphism information content values ranged from 0.15 to 0.79 in Ongole and from 0.13 to 0.80 in Deoni. The F-statistics revealed moderate inbreeding within the breeds and a close relationship between the two breeds studied.
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