EVALUATION OF PROBIOTIC POTENTIAL OF DIFFERENT MICROBIAL STRAINS ISOLATED FROM FOOD MATRICES AND THEIR USE IN FORMULATING FUNCTIONAL HEALTH FOODS
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Date
2018-01
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation was carried out to isolate most efficient potential probiotic
microorganisms from rare and unexplored food sources, their screening, identification, safety assessment,
evaluation of probiotic attributes, metabolic fingerprinting and microencapsulation using different matrices
as well the development of novel nutraceutical products. In total 138 bacterial and 37 yeasts isolates were
obtained from fermented food sources. Out of all, 7 bacterial and 2 yeasts isolates were screened by
bit/disc method on the basis of their broad inhibitory spectrum against tested pathogens. These isolates
were identified as JSi9- Lactobacillus plantarum |MF443877|, S1-Lactobacillus pentosus |MF443879|,
KC6 -Lactobacillus rhamnosus |MF443875|, S1(a)- Lactobacillus paracasei |MF443873|, Si-Enterococcus
durans |MF443876|, Sam1-Lactobacillus paraplantarum |MF443880|, PB- Brevibacillus parabrevis
|MF443878|, F3 -Debaroymyces hensanii |MF588674| and F6 -Candida psychrophila |MF616363| by 16S r
RNA gene technique. These screened isolates were further examined for their probiotic potential viz.
acidity tolerance, bile tolerance, autoaggregation, co-aggregation, hydrophobicity, BSH activity,
antioxidant activity, bacteriocin production, siderophore production, antimicrobial potential,
exopolysaccharides and β-galactosidase production, compatibility and cumulative probiotic potential. All
the nine screened isolates found to be highly acidity tolerant strains with 97.07-55.55 % survival rate at pH
3 for 3 h. These nine isolates were able to resist high bile salt concentration i.e. 2.0% with 68.82 -83.57%
survival rate for 8 h. All the six isolates exhibited good autoaggregation capacity i.e. greater than 40% after
5 h and showed strong hydrophobicity towards xylene i.e. > 40%. All the isolates showed adhesion
potential to mammalian epithelial cell lines. All the nine isolates showed strong antioxidant activity,
Metabolic fingerprinting was done to elucidate the total metabolic profile of isolates Lactobacillus
plantarum JSi9, Lactobacillus pentosus S1, Lactobacillus rhamnosus KC6, Lactobacillus paracasei S1(a)
and Lactobacillus paraplantarum Sam1. Microencapsulation of screened probiotic isolates was done using
different matrices. The entire screened nine isolates were qualified with high cumulative score of 100%
and had been used to prepare probiotic nutraceutical products i.e functional juice, probiotic enriched
functional apricot and plum fruit bar and probiotic enriched soy ice-crem. Hence, this study affirms the
potential use of probiotic microorganisms in the development of new therapeutic functional foods and
nutraceutical preparations to impart goodness to health.
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