Biomarkers of Oxidative Stress and Therapeutic Efficacy of Antioxidants in Donkeys in Varying Ambient Temperatures
| dc.contributor.advisor | Anil Ahuja | |
| dc.contributor.author | Ramesh Kumar | |
| dc.date.accessioned | 2017-01-03T15:09:09Z | |
| dc.date.available | 2017-01-03T15:09:09Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | Twelve adult indigenous donkeys were taken in study; packing exercise was given for two hours (walking with weight of 50% of body weight on back) and given every 9th day of the season. The study was carried out in spring, summer, rainy, autumn and winter seasons of the year. Spring season was kept as control season to study the effect of ambient temperature variation on biomarkers of oxidative stress. Antioxidant supplementation containing lutein, lycopene and vitamin E were given 0.5, 0.5 and 5 mg per kg body weight each in soya oil @ 10 ml/100 kg body weight for 45 days in each season. The donkeys were divided in two groups, group (i) treatment and group (ii) control; equally 6 donkeys were kept in each group. Blood levels of malondialdehyde, catalase, superoxide dismutase and reduced glutathione were measured as biomarkers of oxidative stress. Blood levels of pre exercise malondialdehyde were 18.47±0.89, 28.01±1.24, 27.32±1.80, 19.73±0.95, and 16.79±0.91nanomol/ml in spring, summer, rainy, autumn and winter season respectively in control group and post exercise 19.66±1.00 33.75±1.72, 30.30±2.03, 20.63±0.99, 18.12±0.97nanomol/ml in spring, summer, rainy, autumn and winter, respectively. Blood malondialdehyde was increased significantly after exercise. Seasonal variation of malondialdehyde indicated rise of oxidative stress with increase in ambient temperature, humidity and day length. Catalase, SOD, reduced glutathione and vitamin C, which are the part of antioxidant defence mechanism also found significantly low in summer and rainy season in compare to control spring season. These biomarkers (CAT, SOD, GSH, vitamin C) were at their peak levels in winter season. Following exercise there was compensatory increase in the level of catalase, SOD and reduced glutathione irrespective of season. There was increase in the serum concentration of carotene (control;2.47±0.12and treatment2.82±.09 mg/litre in spring) and vitamin E (control;2.03±0.08 and treatment 2.30±0.07 mg/litre in spring) in treatment group in due to supplementation with vitamin E and carotenes (lutein and lycopene). While serum vitamin A concentration did not change after supplementation. Serum vitamin A concentration was also not affected by exercise while serum vitamin E and C were decreased following exercise. There was no seasonal and post exercise variation in serum vitamin A concentration, it might be due to storage reserve of vitamin A in liver. Exercise has significantly increased activity of serum creatinine kinase (CK) in both groups in each season, there was no significant difference observed in treatment and control group and no significant seasonal variation observed in CK activity. There was significant seasonal variation in serum cortisol concentration with peak in summer and rainy season relates the direct impact of high ambient temperature on stress. Significantly low level of cortisol was observed in antioxidant supplemented group. It was concluded from the study that there is significant seasonal variation in biomarkers of oxidative stress in donkeys.Oxidative stress in donkeys increases with rise in ambient temperature, humidity and day length and antioxidant supplementation is effective to reduce oxidative stress in donkeys in hot and humid environmental conditions.Exercise induces oxidative stress in donkeys, which cannot be prevented with lutein, lycopene and vitamin E combination in the doses used in the present study. | en_US |
| dc.description.sponsorship | Rajasthan University of Veterinary and Animal Sciences, Bikaner - 334001 | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/94194 | |
| dc.language.iso | en | en_US |
| dc.publisher | Rajasthan University of Veterinary and Animal Sciences, Bikaner - 334001 | en_US |
| dc.sub | Veterinary Medicine | en_US |
| dc.subject | enzymes, genotypes, planting, genetics, zingiber officinale, ginger, alleles, locus, polymorphism, electrophoresis | en_US |
| dc.title | Biomarkers of Oxidative Stress and Therapeutic Efficacy of Antioxidants in Donkeys in Varying Ambient Temperatures | en_US |
| dc.type | Thesis | en_US |