Studies on molecular mechanisms underlying rhizobia promiscuity in different Khariflegumes

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dc.contributor.advisorGera, Rajesh
dc.contributor.authorRana, Barkha
dc.date.accessioned2021-08-13T04:26:12Z
dc.date.available2021-08-13T04:26:12Z
dc.date.issued2020
dc.description.abstractMungbean, mothbean, cowpea, clusterbean, urdbean and pigeonpea are economically important kharif legumes commonly grown in rain-fed areas particularly South-West Haryana and Rajasthan state. It is possible to improve its symbiotic property for different kharif legumes through identification and manipulation of genetic factors that controls the promiscuity of Rhizobium. So the present investigation was undertaken to study molecular mechanism underlying rhizobia promiscuity in different kharif legumes. A total of 10 rhizobial isolates along with three rhizobial mutants were obtained from Molecular Microbiology laboratory of Microbiology Department that were retrieved from arid and hyper-arid zones of South-West Haryana and Rajasthan state. All selected rhizobial isolates were checked for their authenticity in six different kharif legumes after 45 days of growth under sterilized conditions. Promiscuous rhizobial mutants (PKR177m30, PKR188m85 and PKR194m93) have the ability to nodulate all six kharif legumes with high nodulation efficiency. Evaluation of these isolates for cellulase activity both on CMC agar plates and in liquid broth revealed that promiscuous rhizobial mutants showed highest cellulase activity both on CMC agar plates and in liquid broth as compared to other rhizobia. Molecular mechanism of rhizobial promiscuity was studied by amplifying and detecting relevant nodulation genes such as nodD, nodT, nodXandnolA in different rhizobia. PCR conditions were optimized to amplify a particular gene. All the 13 selected rhizobial isolates showed the presence of nodD and nodX genes, whereas amplification of nodT was observed in the isolates capable of nodulating 6, 5 and 4 different legumes. However, the expression of nolA gene was fairly good with single amplified PCR product in the rhizobia capable of nodulating 6, 5 and 4 different kharif legumes, amplification of nolA with single PCR amplified product of 672 bp was observed in isolates nodulating 6, 5 and 4 different while rest of the isolates showed poor expression of nolA gene with multiple bands 672 & 323bp. The mutant PKR188m85 performed better in terms of nodulation efficiency and plant growth parameters in pigeonpea, mungbean, clusterbean and mothbean whereas PKR177m30 performed better in cowpea and urdbean followed by PKR188m85 than their respective parent and reference strains under sterilized conditions.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810171830
dc.keywordsRhizobium, Promiscuous, Authenticity, Nodulation, Cellulase, Mechanismen_US
dc.language.isoEnglishen_US
dc.pages49 + viien_US
dc.publisherCCSHAU, Hisaren_US
dc.subMicrobiologyen_US
dc.themeStudies on molecular mechanisms underlying rhizobia promiscuity in different Kharif legumesen_US
dc.these.typeM.Scen_US
dc.titleStudies on molecular mechanisms underlying rhizobia promiscuity in different Khariflegumesen_US
dc.typeThesisen_US
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