STUDIES ON THE ROLE OF TATA BOX BINDING PROTEIN (TBP) ASSOCIATED FACTORS (TAFs) FROM FINGER MILLET IN REGULATING ABIOTIC STRESS ACCLIMATION RESPONSE
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Date
2017-08-05
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Plants in nature often are exposed to a wide range of stress. To cope with these
stress conditions, plants evolved diverse mechanisms. Stress acclimation in plants
involves activation of many stress responsive upstream and downstream genes. In finger
millet (Eleusine coracana) drought specific transcriptome analysis indicated the
importance of basal transcription factors in transcriptional stability under stress
conditions. In a previous study, a stress responsive basal regulatory protein, called TBP
associated factor (TAF6), which is a component of TFIID complex, was cloned from
finger millet and partially characterised. In the present investigation, an attempted was
made to examine the stress responsiveness of other TAFs and validate TAF6. For 15
different TAFs, e-northern analysis was carried out using Arabidopsis and rice expression
data, to select abiotic stress related TAFs. This in-silico expression analysis revealed that
TAF2, TAF4, TAF6, and TAF13 are highly stress responsive, which were shortlisted for
the expression studies in finger millet. Semi-quantitative RT-PCR analysis indicated that
the select TAFs are induced in finger millet leaf tissue under drought. The expression of
TAF13 and TAF4 was significantly higher under stress as evidenced by quantitative RTPCR.
In seedlings, these TAFs were up-regulated under NaCl and Poly Ehtelyne Glycol
(PEG) PEG-induced stresses. Arabidopsis transgenic plants developed in an earlier study,
to over-express EcTAF6 cloned from finger millet were screened and subjected for
molecular characterization. The transgenic plants carrying EcTAF6 exhibited superior
phenotype compared to the wild type under mannitol- and NaCl-induced stresses
indicating the relevance of the TAF in stress acclimation.
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