Clinical and Molecular Diagnosis of Johne’s Disease in a Jamunapari Goat – A Case Report
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Date
2017
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Abstract
A 2 year old male Jamunapari goat weighing 30 kilograms was presented
with complaints of chronic diarrhea, progressive weight loss and poor
response to therapy. Faecal sample was collected aseptically and subjected
for the presence of Mycobacterium avium subspecies paratuberculosis
(MAP) by Ziehl-Neelsen staining and polymerase chain reaction (PCR).
Ziehl – Neelsen staining (ZN) revealed the presence of clumped acid fast
bacilli in the faecal smears. For further differencing between Acid fast
bacilli from nonacid fast MAP confirmation, DNA was extracted from
faecal sample and subjected to PCR by using specific primers targeting IS
900 gene sequence of MAP and confirmed JD by detection of 413 bp PCR
product.
Description
TNV_IJCMAS_2017_6(10)119-122
Keywords
Veterinary Science