VALIDATION OF NEUTROPHILS THRESHOLD IN ENDOCERVICAL SMEARS TO DEFINE SUBCLINICAL ENDOMETRITIS IN CROSSBRED COWS
| dc.contributor.advisor | MOULI KRISHNA, K(MAJOR) | |
| dc.contributor.advisor | MUTHA RAO, M | |
| dc.contributor.advisor | PADMAJA, K | |
| dc.contributor.author | SHANMUKHI, S | |
| dc.date.accessioned | 2018-11-30T11:02:47Z | |
| dc.date.available | 2018-11-30T11:02:47Z | |
| dc.date.issued | 2016-10 | |
| dc.description | THESES | en_US |
| dc.description.abstract | ABSTRACT : The objectives of the present study was to evaluate the technique of reading the cytological smears to diagnose subclinical endometritis and validate the neutrophils threshold in relation to fertility in crossbred cows. A total of 161 consisting of 120 normal and 41 repeat breeding crossbred cows, which were brought to nearby rural veterinary institutions were utilized. For cytological studies, discharge from cervix was aspirated at standing estrus and smears were evaluated by three different counting methods i.e., mean count from five MF (A), ten MF (B) and hundred cells (C). The mean neutrophils count was 0.67±0.34 and 1.05±0.61/MF; 0.73±0.36 and 0.90±0.54/MF; 6.11±1.9 and 6.2±3.3% from method A, B and C in normal and repeaters, respectively and did not differ significantly. The neutrophils count in cytological smears was found unassociated with number of inseminations, parity and lactation length both in normal and repeaters. The mean total cells count was 7.7±0.52 and 10.5±1.3 / MF; 6.76±0.51 and 8.09±1.09 / MF from method A and B in normal and repeater cows, respectively and differed significantly. The total cells count did not differ significantly between positive and negative cows for pregnancy from normal and repeaters in method A and B. Based on fertility in normal cows and repeaters the mean neutrophils count from method A and B did not differ significantly in positive and negative cows, respectively. While, in normal group the mean count from method C was 1.14±0.38% and 10.2±2.94% in positive and negative cows for pregnancy, respectively and differed significantly (p≤ 0.01) whereas in repeaters the mean neutrophils count from method C was 0.62±0.35 and 10.1±5.5% in positive and negative cows for pregnancy, respectively and did not differ significantly. The frequency distribution in normal positive cows 44.03% (48/109) and 2.75% (3/109) and in negative cows 42.2% (46/109) and 11%(12/109) whereas in repeaters 41% (16/39) and 0% (0/39) in positive cows and 46.15% (18/39) and 12.8% (5/39) in negative cows at 0-5% and ≥ 6% neutrophils threshold, respectively and differed significantly (p≤ 0.01) at ≥ 6% neutrophils threshold both in normal and repeater group. The specificity of method A and B was lower in comparison to method C and there was no consistency in neutrophils count between method A and B. It was concluded that the method of taking mean cells count per one MF cannot be validated and the neutrophils threshold of ≤ 5% could be considered as cut off level for establishing the cytological evidence of presence of uterine infections and there by predicting the fertility in those animals. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810084907 | |
| dc.keywords | NEUTROPHILS THRESHOLD;ENDOCERVICAL SMEARS;SUBCLINICAL ENDOMETRITIS;CROSSBRED COWS; VALIDATION | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 111 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Gynaecology and Obstetrics | en_US |
| dc.subject | null | en_US |
| dc.theme | VALIDATION OF NEUTROPHILS THRESHOLD IN ENDOCERVICAL SMEARS TO DEFINE SUBCLINICAL ENDOMETRITIS IN CROSSBRED COWS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | VALIDATION OF NEUTROPHILS THRESHOLD IN ENDOCERVICAL SMEARS TO DEFINE SUBCLINICAL ENDOMETRITIS IN CROSSBRED COWS | en_US |
| dc.type | Thesis | en_US |