Generation of interspecific hybrids in chickpea ( Cicer Arietinum L.) and their confirmation through morphological and molecular analyses

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dc.contributor.advisorAjinder Kaur
dc.contributor.authorRitika
dc.date.accessioned2022-11-11T14:32:11Z
dc.date.available2022-11-11T14:32:11Z
dc.date.issued2021
dc.description.abstractThe present study was conducted on ―Generation of interspecific hybrids in chickpea (Cicer arietinum L.) and their confirmation through morphological and molecular analyses‖. A total of 12 different crosses (using 546 flower buds) were attempted (C. arietinum  C. reticulatum: L552 × EC396375, PBG7 × EC396375, PBG7 × EC426536, PBG7 × ILWC292, GPF2 × EC396375, PDG3 × ILWC292; C. reticulatum  C. pinnatifidum: ILWC141 × C. pinnatifidum 49; C. reticulatum  C. reticulatum: PB64 × ILWC292; C. arietinum  C. bijugum: L552 × ILWC0, PDG3 × ILWC0; C. arietinum  C. judaicum: L552 × C. judaicum 185B, PDG3 × C. judaicum 185B). A mixture of plant growth regulators (GA3 120 ppm + NAA 30 ppm + Kin 15 ppm) were applied to the base of pedicels of crossed flowers to prevent flower drop and enhance pod setting. A total of 76 ovules were rescued and cultured, however no plants were obtained. A total of 42 seeds were obtained from crosses left under natural conditions that were sown to raise 27 putative F1 plants (C. arietinum  C. reticulatum: 25 plants, C. reticulatum  C. pinnatifidum: one plant, C. reticulatum  C. reticulatum: one plant). The remaining cross combinations belonging to C. arietinum  C. bijugum and C. arietinum  C. judaicum did not result in any F1 plant. Twenty two plants from C. arietinum  C. reticulatum and one each from C. reticulatum  C. pinnatifidum and C. reticulatum  C. reticulatum crosses were proved to be hybrids through morphological traits viz. plant height, growth habit, number of secondary branches, number of pods and seeds per plant, and seed yield. Eighteen plants obtained from crosses C. arietinum  C. reticulatum and one plant from C. reticulatum  C. pinnatifidum were proven to be hybrids on the basis of molecular data of five polymorphic SSR markers, namely TA2, TA43, TA89, TA116 and TS45. The molecular and morphological data of F1 plants corroborated with each other to support hybridity of 19 F1 plants. Generation of maximum number of plants from C. arietinum  C. reticulatum cross revealed that genetic variability found in C. reticulatum can be utilized for the improvement of cultivated chickpea. All plants were obtained without the use of embryo rescue.en_US
dc.identifier.citationRitika (2021). Generation of interspecific hybrids in chickpea ( Cicer Arietinum L.) and their confirmation through morphological and molecular analyses (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189789
dc.keywordsSSR, interspecific crosses, F1 hybrids, ovule culture, plant growth regulators, Cicer, MS mediumen_US
dc.language.isoEnglishen_US
dc.pages68en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemGeneration of interspecific hybrids in chickpea ( Cicer Arietinum L.) and their confirmation through morphological and molecular analysesen_US
dc.subAgricultural Biotechnologyen_US
dc.themeGeneration of interspecific hybrids in chickpea ( Cicer Arietinum L.) and their confirmation through morphological and molecular analysesen_US
dc.these.typeM.Scen_US
dc.titleGeneration of interspecific hybrids in chickpea ( Cicer Arietinum L.) and their confirmation through morphological and molecular analysesen_US
dc.typeThesisen_US
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