Generation of interspecific hybrids in chickpea ( Cicer Arietinum L.) and their confirmation through morphological and molecular analyses
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
The present study was conducted on ―Generation of interspecific hybrids in chickpea (Cicer arietinum L.) and their confirmation through morphological and molecular analyses‖. A total of 12 different crosses (using 546 flower buds) were attempted (C. arietinum C. reticulatum: L552 × EC396375, PBG7 × EC396375, PBG7 × EC426536, PBG7 × ILWC292, GPF2 × EC396375, PDG3 × ILWC292; C. reticulatum C. pinnatifidum: ILWC141 × C. pinnatifidum 49; C. reticulatum C. reticulatum: PB64 × ILWC292; C. arietinum C. bijugum: L552 × ILWC0, PDG3 × ILWC0; C. arietinum C. judaicum: L552 × C. judaicum 185B, PDG3 × C. judaicum 185B). A mixture of plant growth regulators (GA3 120 ppm + NAA 30 ppm + Kin 15 ppm) were applied to the base of pedicels of crossed flowers to prevent flower drop and enhance pod setting. A total of 76 ovules were rescued and cultured, however no plants were obtained. A total of 42 seeds were obtained from crosses left under
natural conditions that were sown to raise 27 putative F1 plants (C. arietinum C. reticulatum: 25 plants, C. reticulatum C. pinnatifidum: one plant, C. reticulatum C. reticulatum: one plant). The remaining cross combinations belonging to C. arietinum C. bijugum and C. arietinum C. judaicum did not result in any F1 plant. Twenty two plants from C. arietinum C. reticulatum and one each from C. reticulatum C. pinnatifidum and C. reticulatum C. reticulatum crosses were proved to be hybrids through morphological traits viz. plant height, growth habit, number of secondary branches, number of pods and seeds per plant, and seed yield. Eighteen plants obtained from crosses C. arietinum C. reticulatum and one plant from C. reticulatum C. pinnatifidum were proven to be hybrids on the basis of molecular data of five polymorphic SSR markers, namely TA2, TA43, TA89, TA116 and TS45. The molecular and morphological data of F1 plants corroborated with each other to support hybridity of 19 F1 plants. Generation of maximum number of plants from C. arietinum C. reticulatum cross revealed that genetic variability found in C. reticulatum can be utilized for the improvement of cultivated chickpea. All plants were obtained without the use of embryo rescue.
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Ritika (2021). Generation of interspecific hybrids in chickpea ( Cicer Arietinum L.) and their confirmation through morphological and molecular analyses (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.