CLONING AND EXPRESSION OF IMMUNOGENIC ANTIGENS OF Mycobacterium avium subsp. paratuberculosis
Abstract
Paratuberculosis (pTB) is a fatal inflammatory disease caused by
Mycobacterium avium subsp. paratuberculosis (MAP) affecting both wild and
domestic ruminants.Nospecificand sensitive diagnostic assay is available due to the
cross reactivity with the strains of vast mycobacterium family. Development of
diagnostics for MAPwill require a cocktail of antigens due to therange of
heterogeneous Major histocompatability complex(MHC) molecules and due to
differentiated antigen expression patterns of MAP. Recombinant antigens for the
Mycobacterium avium subsp. paratuberculosisgenes namely MAP4_0900 encoding
34kDa B cell epitope, MAP specific IS900 - Nir Aencoding T cell epitope, MAP4_
2856 (ESXO-ESAT) encoding early secretory ESAT 6 like protein,MAP4_1239c
encoding virulence factor MmpL were cloned and expressed in E.coli. The gene
inserts for the selected genes were amplified byPolymerase chain reaction (PCR) with
specific primers designed for site directional cloning. A fusion gene encoding 28
immunogenic peptides of nine genes, Ag85A,Ag85B, Ag85C,P35-35kDa, Serine
Protease, AhpC, AhpD, GroE1, Hsp20 was synthesised and obtained as a pUC 57
vector insert.
Description
TNV_TH_2018_DPV15003
Keywords
Veterinary Science