STUDIES ON IN VITRO MATURATION OF FOLLICULAR OOCYTES OF BUFFALOES
| dc.contributor.advisor | SOLMON RAJU, K.G(MAJOR) | |
| dc.contributor.advisor | SADASIVA RAO, K | |
| dc.contributor.advisor | CHANDRA SEKHAR, E.L | |
| dc.contributor.author | KULA SEKHAR, TIRUKKOVALLURI | |
| dc.date.accessioned | 2018-10-26T06:26:44Z | |
| dc.date.available | 2018-10-26T06:26:44Z | |
| dc.date.issued | 2008-01 | |
| dc.description.abstract | ABSTRACT : A total number of 498 ovaries were procured from municipal slaughter house, Hyderabad soon after the slaughter of the buffaloes. The ovaries were subjected to slicing, aspiration and post aspiration slicing in order to retrieve the oocytes. The oocyte retrieval rate was 73.68, 43.61 and 47.91 per cent in slicing, aspiration and post aspiration slicing methods, respectively. The oocyte recovery per ovary was 2.91, 1.53 and 1.89 in slicing, aspiration and post aspiration slicing, respectively. The retrieved oocytes were graded into A, B, C and D. The yield of A grade oocytes were 53.92, 42.57 and 46.72 in slicing, aspiration and post aspiration slicing techniques, respectively. The yield of B grade oocytes was 28.87, 20.92 and 29.10, respectively. The yield of C and D grades of oocytes were 15.69 and9.52; 11.27 and 11.24; and 13.93 and 10.25 in slicing, aspiration and post aspiration slicing methods, respectively. The A, B and C grade oocytes were matured in TCM 199, TCM 199 supplemented with PMSG and buffalo follicular fluid (buFF) alone. Significantly higher per cent of morphological maturation was observed in PMSG supplemented media. Significantly higher per cent of grade A and B oocytes were morphologically matured than the grade C oocytes. Basing on the degree of cumulus expansion, the maturation was classified as D0, D1 and D2 cumulus expansion. Significantly higher per cent of A and B grade oocytes attained D2 and D1 degree of cumulus expansion while significantly higher per cent of C grade oocytes retained in D0 degree of cumulus expansion. The per cent of oocytes extruded first polar body was 70.83, 5.55 and 42.50 in PMSG, buFF and TCM 199 media. The per cent of oocytes not extruded the polar body was 47.50, 22.99 and 70.30 in TCM 199, PMSG and buFF culture media respectively. Higher per cent of oocytes degenerated in buFF culture media than the TCM 199 and PMSG media in the present study. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810082199 | |
| dc.keywords | FOLLICULAR OOCYTES;BUFFALOES;IN VITRO; MATURATION | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 129 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Animal Reproduction and Gynecology | en_US |
| dc.subject | null | en_US |
| dc.theme | STUDIES ON IN VITRO MATURATION OF FOLLICULAR OOCYTES OF BUFFALOES | en_US |
| dc.these.type | M.V.Sc. | |
| dc.title | STUDIES ON IN VITRO MATURATION OF FOLLICULAR OOCYTES OF BUFFALOES | en_US |
| dc.type | Thesis | en_US |