EFFECT OF PRECONDITIONING TREATMENTS, SIZE OF CUTTINGS, PLANT GROWTH PROMOTING RHIZOBACTERIA AND IBA ON ROOTING IN CUTTINGS OF APPLE ( Malus x domestica Borkh.) CLONAL ROOTSTOCK MERTON 793
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Date
2013
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ABSTRACT
The present investigation entitled “Effect of preconditioning treatments, size of cuttings, plant growth promoting rhizobacteria and IBA on rooting in cuttings of apple (Malus x domestica Borkh.) clonal rootstock Merton 793” were undertaken in net house condition of Fruit Nursery Block of Department of Fruit Science, Dr Y.S. Parmar University of Horticulture and Forestry Nauni, Solan H.P. during 2012-13. Four experiments were laid out in randomized block design. In Experiment I cuttings of length ranging from 15 cm to 45 cm and diameter ranging from 0.75 to 1.50 cm, in order to study the effect of different size of cuttings on rooting in cuttings. The results revealed that the cuttings with treatment combination of L – 35 cm and D – 1.25 – 1.50 cm recorded the highest rooting of cuttings (65 %), number of primary roots (5.00), length of primary roots (28.43 cm), diameter of primary roots (3.25 mm), fresh weight of roots (3.67 g) and dry weight of roots (2.59 g). In Experiment II, the studies on controlled preconditioning storage conditions (growth chamber) and IBA treatments on rooting of cuttings of apple clonal rootstock Merton 793 was carried out. The cuttings stored for 7 days along with IBA 2500 ppm treatment recorded highest rooting percentage, number of primary roots, length and diameter of primary roots, total root length, fresh and dry weight of roots recording 46.67 %, 4.60, 28.60 cm, 2.63 mm, 5.61 m, 2.89 g and 1.83 g, respectively. In Experiment III, the studies on field preconditioning storage conditions (polyhouse) and IBA treatments on rooting of cuttings of apple clonal rootstock Merton 793 was also carried out. The cuttings stored for 7 days along with 2500 ppm IBA treatment resulted in highest rooting percentage of 53.33 % and cuttings stored for 14 days along with IBA 2500 ppm resulted in maximum length and diameter of roots recording 19.88 cm and 2.58 mm, respectively. In Experiment IV, the preliminary studies on the effect of different strains of PGPR alone and in combination with IBA at 1000 ppm on rooting were carried out. The PGPR strains (RG (1)3 – Bacillus sp), B6 – Bacillus licheniformis and R3 (3) – Sirretia sp alone failed to induce any rooting response in cuttings, whereas, the PGPR strains (RG (1)3 – Bacillus sp), B6 – Bacillus licheniformis and R3 (3) – Sirretia sp using IBA 1000 ppm recorded some improvement in rooting recording 10, 15 and 5 % rooting respectively. However IBA 2500 ppm (kept as control) the standard concentration of IBA records the maximum rooting of cuttings of Merton 793, recording 65.00 % rooting. In brief, cuttings of 35 cm length and 1.25 – 1.50 cm diameter were most suitable for propagation. Preconditioning treatment of 7 days in controlled incubator (22ºC) or 7 to 14 days in field condition with IBA 2500 ppm were suitable for helping in rooting of cuttings in Merton 793. PGPR strains failed to induce rooting in cuttings. However, PGPR strains in combination with 1000 ppm induced some rooting in cuttings.