PROTEOMIC PROFILING OF OVARIAN FOLLICULAR FLUID FROM NORMAL AND PROLONGED FOLLICULAR DOMINANCE IN DEONI COWS
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Date
2022
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ICAR-SRS-NDRI, KARNAL
Abstract
Dairying is an integral part of animal husbandry, and efficient reproduction in dairy cattle makes a distinct contribution to the socio-economic growth of the country. Prolongation of follicular dominance is one of the conditions associated with disconcerted follicular dynamics that result in substantial economic losses through low reproductive efficiency. Despite the fact that there is a lot of research available on the mechanisms of prolonged follicular dominance and oocyte viability, the proteomic alterations in the microenvironment of the prolonged follicle remain unknown. In this regard, we investigated the ultrasonographic characteristics of follicles as well as the proteomic alterations in follicular fluid from normal (NDFF) and prolonged dominant follicles (PDFF). Prolonged dominance was induced in Deoni cows (n = 6) by placing CIDR (previously used for 7 days) intravaginally from day 4 to 8 of estrus, with a luteolytic dose of PGF2 on day-6 and day-7resulting in subluteal progesterone concentration. Ultrasonographic examination revealed no significant difference in the size and growth pattern of normal and prolonged dominant follicles. The concentration of serum progesterone in the prolonged follicular dominance group was significantly higher (P<0.05) than in the normal follicular dominance group on the day of transvaginal follicular aspiration (day-8). Furthermore, global proteomic analysis of follicular fluid detected 217 proteins in the Deoni cow, with the majority of the identified proteins involved in 21 pathways, 42 molecular functions, and 106 biological processes. Complement and coagulation cascades (22.8%) and cholesterol metabolism (4.68%) were the major pathways in which identified proteins were involved. In comparison to NDFF, 26 proteins were dysregulated in PDFF. Among them, 15 proteins showed upregulation, and 11 proteins showed downregulation. Proteins involved in complement and coagulation cascades, and vitamin digestion and absorption were found to be dysregulated. In this study, the ovarian follicular characteristics were not different in cows experiencing prolonged follicular dominance from those of normal follicular dominance. However, the expression of proteins involved in inflammation, oocyte metabolism, and ovulation of follicles was dysregulated in the follicular fluid of cows with prolonged follicular dominance, which might be responsible for delayed ovulation.