EFFECT OF FOLLICULAR FLUID SUPPLEMENTATION ON IN-VITRO MATURATION AND DEVELOPMENTAL RATE OF BUFFALO OOCYTES

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dc.contributor.authorSatheshkumar, S.
dc.contributor.authorPriya, M. Shalini
dc.contributor.authorBrindha, K.
dc.contributor.authorSakthivel, S.
dc.contributor.authorKumanan, K.
dc.contributor.authorTANUVAS
dc.date.accessioned2018-04-02T10:38:07Z
dc.date.available2018-04-02T10:38:07Z
dc.date.issued2017-06
dc.descriptionTNV_IJAH_2017_56(1)31-36en_US
dc.description.abstractThe effect of supplementation of follicular fluid (FF) at 5.0% concentration in in vitro maturation (IVM) media on in vitro production of buffalo embryos was studied. Oocytes were aspirated from slaughter house derived ovaries and graded. Cultutarable quality oocytes were randomly cultured in IVM media with FF (n = 334) and without FF (n = 228) and subjected to maturation. The maturation rate, cleavage rate and embryo developmental rate was recorded in both the groups. The maturation, cleavage, morula and blastocyst rates (78.2 ± 1.8, 41.2 ± 1.3, 15.2 ± 0.7 and 7.7 ± 0.9 % respectively) were significantly (P < 0.01) higher when oocytes were matured in media supplemeted with FF than controls (55.3 ± 1.9, 28.7 ± 1.5, 6.7 ± 0.8 and 2.2 ± 0.8 % respectively). Thus, it could be concluded that supplementation of FF (5.0 %) to IVM media would benefit the maturation, cleavage and embryo development in buffalo IVF programme.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810042868
dc.keywordsBubaline oocytes, Embryo development, IVM with or without follicular fluid *Corresponding Author 1Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Tamilnadu Veterinary and Animal Sciences University, Orathanadu – 614 625, Thanjavur Follicle isen_US
dc.language.isoenen_US
dc.pages31-36en_US
dc.publisherWest Bengal Veterinary Associationen_US
dc.relation.ispartofseries;1
dc.subjectAnimal Healthen_US
dc.titleEFFECT OF FOLLICULAR FLUID SUPPLEMENTATION ON IN-VITRO MATURATION AND DEVELOPMENTAL RATE OF BUFFALO OOCYTESen_US
dc.title.alternativeIndian Journal of Animal Healthen_US
dc.typeArticleen_US
dc.volume56en_US
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