PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF ACTIVATION TAGGED MUTANTS OF RICE FOR FLOWERING, GROWTH, YIELD AND SEED CHARACTERISTICS BESIDES A FEW DROUGHT ADAPTIVE TRAITS
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Date
2014-09-10
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University of Agricultural Sciences, Bengaluru
Abstract
Yield improvement under water limited conditions can be best achieved by
pyramiding stress adaptive traits onto agronomically superior background either by
conventional/ molecular breeding or by transgenic approach. Besides these, gain-infunction
activation tagging approach can also be followed to generate mutant populations
with enhanced expression of certain genes. However, the process is random and hence,
warrants the generation of large number of independent events and later, phenotype them
for trait of interest. With this background, T3 generation activation tagged mutants of
rice developed earlier in JBT 36/14, a rice cultivar adapted to aerobic condition was
characterized for growth and productivity besides flowering and a few drought traits.
The activation tagged mutants showed large variations in traits associated with
growth and productivity such as plant height, tiller number, productive tiller number,
days to 50% flowering, seed yield, TDM, test weight and HI. In fact, some of the
mutants showed significantly higher TDM and seed yield compared to wild type. Further,
as against highly photosensitive nature of wild type, some mutants with less
photosensitivity noticed. In addition, mutants with desirable plant architecture such as
erect tillering habit with erect and broader leaves that are lacking in wild type observed.
Significant variations in root traits, cellular level tolerance and seed colour were also
noticed and a few mutants better than wild type in root traits identified. Further, photoinsensitive
mutants identified were found to be PCR positive and flanking sequence
analysis of one of the photo-insensitive mutants (No. 212) revealed that, integration of
enhancer element has occurred on chromosome 11 of the locus LOC_Os11g30484 which
codes for Zinc finger protein. From this, it appears that, the zinc finger protein somehow
regulates the gene controlling flowering in rice.
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