DEVELOPMENT OF MAGIC POPULATION AND TAGGING OF RESISTANCE TO MAJOR DISEASES IN MAIZE (Zea mays L.)
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Date
2022-06-24
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University of Agricultural Sciences, Bangalore
Abstract
Northern corn leaf blight (NCLB) and Sorghum downy mildew (SDM) of maize are serious constraints to maize production worldwide. An attempt was made to develop Multiparent advanced generation intercross (MAGIC) population segregating for NCLB and SDM from the eight diverse founder parents (PDM-4341, CML451, CM202,CM212, SKV50, CAL1443, CAL1518 and VL109545) which were chosen based on SSR diversity and phenotypic response to multiple diseases. The MAGIC population was developed based on simple funnel crossing scheme to obtain 288 F3 progenies and they were phenotyped during Summer and kharif 2020 using alpha-lattice design in the artificial disease screening nurseries for NCLB and SDM at ZARS, Mandya. Analysis of variance among F3 progenies showed significant variation for NCLB and SDM disease incidence. The per cent disease severity for NCLB and per cent disease incidence for SDM showed high PCV and high heritability. Out of 288 F3 progenies, 14 F3 progenies were resistant to NCLB whereas, four progenies were resistant to SDM. Genome wide association study was conducted using 90 SSR markers to detect the genomic regions controlling resistance to NCLB and SDM. PCA analysis suggested an absence of unique groupings within the population. Based on the results of Mixed linear model (MLM) four SSRs were found associated with resistance to NCLB and one SSR marker (bnlg109) for SDM. BLAST analysis revealed that five SSRs associated with NCLB and one SSR marker with SDM were found overlapped within annotated genes (Callose synthase 7, Non-specific lipid-transfer protein, Homeodomain leucine zipper family IV protein, BTB/POZ domain-containing protein NPY2, AP2-EREBP-transcription factor 197 and Fbox/LRR-repeat protein) with plant defence mechanism.