In ovo delivery of Lactobacillus acidophilus on the growth and immune response of commercial broiler chicken
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Chemical Studies
Abstract
A nutritional trial was conducted to investigate the effect of in ovo injection of Lactobacillus acidophilus to 18 days old broiler chicken embryo on the growth performance and humoral immune response of commercial broilers. In ovo injection was carried out on 18th day of incubation, out of total 720 broiler hatching eggs, 144 eggs served as non injected control (T1), 144 eggs served as sham control and the remaining 432 eggs (144 for each treatment group) were injected with 0.2 ml of 1x106 Lactobacillus acidophilus (T3), 0.2 ml of 1x109 Lactobacillus acidophilus (T4) and 0.2ml of 1x1012 Lactobacillus acidophilus (T5). The positive control group was injected with 0.2 ml of 0.9% normal saline solution. At hatch, 480 chicks were randomly selected (96 birds in each treatment) with six replicates of 16 birds each as per treatment wise. Data on hatchability, sixth week weight gain, cumulative FCR, livability and antibody titres against sheep RBC antigen were recorded and statistically analysed. There was no significant difference in percent hatchability among treatment and control groups. The 6th week body weight and weight gain were significantly (P<0.01) affected by in ovo injection of Lactobacillus acidophilus irrespective of concentration. The feed consumption was significantly (P<0.01) more in probiotic injected groups compared to other groups only on second week. The 6th week cumulative FCR was not significantly affected by in ovo infusion of probiotics. The livability was significantly (P<0.05) higher in probiotic infused groups. Antibody titre against SBRC antigen was significantly (P<0.01) increased by in ovo treatments compared to sham and control. The TLR-2 gene was significantly (P<0.01) up regulated in the spleen and caecal tonsil’s of L. acidophilus infused broilers. The results of this trial indicated that the in ovo injection of Lactobacillus acidophilus has got beneficial effect on the growth performance, survivability and immune status of commercial broilers.
Description
TNV_IJCS_2018_6(5)_2433-2436
Keywords
Veterinary Science, Poultry Science