Non-Invasive Fecal Based PCR Assays for Detection of Mouse Parvo Virus and Minute Virus of Mice in Laboratory Mice

Simple item page
dc.contributor.authorSrinivasan, MR
dc.contributor.authorVijay, K
dc.contributor.authorKaruppannan, AK, et al.,
dc.contributor.authorTANUVAS
dc.date.accessioned2021-12-15T11:54:18Z
dc.date.available2021-12-15T11:54:18Z
dc.date.issued2021-11
dc.descriptionTNV_IJAR_2021_55(11)1356-1359en_US
dc.description.abstractABSTRACT Background: Murine parvoviruses are a few of the most common pathogens of laboratory mice posing potential threats to mice colonies and experiments conducted in mice. We initiated this study to develop the fecal based PCR assays as an alternative to serology and to check its feasibility to detect the infections in mice caused by Mouse Parvo Virus (MPV) and Minute Virus of mice (MVM). Methods: Primers targeting the VP2 gene of MPV and MVM were selected and their sensitivity was analysed in tenfold serially diluted gene template in the presence of negative mouse fecal DNA. Selected thirty-seven mice at the age of 6 to 18 weeks randomly and collected blood samples for serology and fecal samples for PCR assays. Result: PCR assays of MPV and MVM detected as low as 0.4 fg of the target plasmid DNA. PCR assays in fecal samples of mice detected the presence of natural infections of MPV and MVM and their respective prevalence was 24% and 30%. Diagnostic sensitivity of MPV and MVM were 74% and 76% respectively. Our findings indicate that the fecal-PCR assay may be a useful, non-invasive and sensitive diagnostic tool in the ante-mortem detection of MPV and MVM in the health monitoring program.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810179339
dc.keywordsFecal PCR, Health monitoring, Minute virus of mice, Mouse parvo virus.en_US
dc.language.isoEnglishen_US
dc.pages1356-1359en_US
dc.relation.ispartofseries;11
dc.subjectVeterinary Scienceen_US
dc.titleNon-Invasive Fecal Based PCR Assays for Detection of Mouse Parvo Virus and Minute Virus of Mice in Laboratory Miceen_US
dc.title.alternativeIndian Journal of Animal Researchen_US
dc.typeArticleen_US
dc.volume55en_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
TNV_IJAR_2021_55(11)1356-1359.pdf
Size:
239.4 KB
Format:
Adobe Portable Document Format
Description:
TNV_IJAR_2021_55(11)1356-1359
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Articles (1.Journal)