Virulence Genes Detection and Antimicrobial Susceptibility of Staphylococcus pseudintermedius Isolates from Canine Skin Infection in Chennai, India
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Date
2018-01
Journal Title
Journal ISSN
Volume Title
Publisher
Council of the National Academy of Sciences
Abstract
Staphylococcus pseudintermedius (SP) is the
major pathogen incriminated in the skin infections of dog.
Identification of SP requires molecular methods. The
incidence of methicillin resistant SP (MRSP) is increasing
worldwide and it is a growing concern in treating pet
animals. The prevalence of SP and MRSP from skin
infections of dog in India has not been studied previously.
Hence, the present study was aimed to isolate SP from
common skin infections of dog in Chennai, India and to
characterize these isolates. A total of 53 SP organisms were
isolated from 91 samples of skin infection accounting for
59 % of isolation rate. Labrador was a major breed from
which isolation was made. Panton–Valentine leucotoxin
(Luk-I) and S. intermedius exfoliative toxin (siet) genes
were detected in all SP isolates but staphylococcal protein
A homologue (spsQ) gene was detected only in 36 % of the
SP isolates. Out of 53 isolates, 17 % were found to be
strong and 19 % to be moderate producers of biofilm and
28 % were classified as MRSP due to possession of the
mecA gene. Most isolates were sensitive to tetracycline and
ciprofloxacin and least sensitive to erythromycin and
trimethoprim/sulphamethaxazole. The authors first time
reported the isolation of MRSP, characterization of SP
isolates by detecting virulence genes, biofilm forming
ability and susceptibility to antimicrobials in Chennai,
India.
Description
TNV_PNAS_2018_88(1)355-361
Keywords
Veterinary Science