Studies on microbe mediated biochemical and molecular mechanism responsible for induced systemic resistance (ISR) and plant growth promotion in Lentil (Lens culinaris Medik.) under stress of pathogenic “Fusarium oxysporum f. sp. lentis
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Date
2020-10-29
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ANDUAT,Kumaraganj,Ayodhya
Abstract
A total of 20 rhizospheric strains were isolated at NA, King’s B and Jension
media. Out of 20, only eight isolates (40 percent) showed antagonistic activity against
Fusarium oxysporum f.spp.lentis. All the antagonistic isolates produced diffusible and
volatile antifungal metabolites. The isolates (B5) and (B8) showed a maximum
antagonism as 77.8% and 70% respectively. All the antagonistic isolates showed
PGPR activity such as phosphate solubilization, Zn solubilization, Siderophore
production ,Ammonia production, HCN and IAA production. On the basis of their
antagonistic and PGPR functionality, out of eight, only two isolates, B5 and B8 were
found to be more effective which were selected for ISR against wilt of lentil and
characterized B5 and B8 strains by 16SrRNA molecular primer. Strain B5 and B8
identified as Bacillus safensis and Bacillus licheniformis respectively. The effect of
these two bio-agents (Bacillus safensis and Bacillus licheniformis spp.) against wilt
phyto-pathogen (Fusarium oxysporum f.sp.lentis ) lentil (Lens culinaris Medik.) was
determined. Two varieties of lentil as resistant (IPL-316) and susceptible (K-75) were
taken for the experiment.
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