Prevalence, oxidative stress and phyto-therapeutic management of subclinical mastitis with special reference to Staphylococcus aureus in buffaloes
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Date
2019-12
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was undertaken to investigate the epidemiology and to determine level of oxidative stress in dairy buffaloes suffering from subclinical mastitis with emphasis on Staphylococcus aureus infection, followed by evaluation of herbal antimicrobial therapy and to compare it with standard antimicrobial therapy based upon antimicrobial sensitivity testing. The overall prevalence of subclinical mastitis recorded in and around Pantnagar was 19.48%. The highest occurrence of subclinical mastitis was observed in high producing 4th to 6th parity buffaloes in their early stage of lactation with more predispositions towards hind-quarters. Staphylococci followed by Streptococci were found to be a major etiological agent. The overall antibiogram of the major bacterial isolates revealed the highest sensitivity to enrofloxacin. PCR assay targeting DNA sequence encoding for 23S rRNA of Staphylococcus aureus was found highly sensitive in rapid identification of S. aureus directly from milk samples as compared to milk culture. There was significant increase in levels of LPO, SOD, CAT, LDH and ALP while significant reduction in GSH was observed in buffaloes with SCM. No significant difference was observed in BUN concentration in buffaloes with SCM which suggests that there was prolonged infection by gram positive bacteria. Micromineral estimation revealed higher serum zinc and iron levels and non-significant increase in serum copper level in buffaloes with subclinical mastitis than that of healthy control. Therapeutic trial revealed the highest efficacy of intramammary infusion of enrofloxacin, followed by Trisama extracts in management of subclinical mastitis in buffaloes which needs further validation through large scale clinical trials.