Assessing Mycobacterium avium subsp. paratuberculosis (MAP) Infection Status in Small Ruminants
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Date
2014-12
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Abstract
The present study was aimed to assess the
Mycobacterium avium subsp. paratuberculosis
(MAP) infection status in small ruminants.
A total of 47 faecal samples inclusive of 23
from sheep and 24 from goats were collected
and subjected to Ziehl-Neelson (Z-N) staining
technique and polymerase chain reaction
(PCR). Out of 23 faecal samples collected from
sheep none of the samples were positive and 1
sample out of 24 faecal samples from goats was positive by Z-N staining technique. Nine out of 23 samples from sheep and 7 out of 24 samples
from goats were positive for MAP infection by
PCR. Sensitivity and specificity of Z-N staining
technique was calculated. Sensitivity and
specificity of Z-N staining technique were of 5.8
and 100 per cent respectively when compared
with PCR. Hence PCR can be used effectively for
assessing the infection status of MAP in small
ruminants.
Description
TNV_IVJ_2014_91(12)74-76
Keywords
Veterinary Science