ISOLATION AND MOLECULAR CHARACTERIZATION OF ANTIMICROBIAL RESISTANT ESCHERICHIA COLI IN PORK PRODUCTION CHAIN IN HASSAN DISTRICT OF KARNATAKA
Loading...
Files
Date
2023-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA
Abstract
The present investigation was carried out to evaluate the occurrence of AMR E.
coli in pork production chain in and around Hassan. Initial survey indicated that majority
of the pig farmers were marginal farmers (65%) and kitchen / hotel waste was the major
source of feed. The major antibiotics used were enrofloxacin followed by
streptopenicillin, tetracycline, fortified procaine penicillin sulphonamides (Co-trimazole)
and antibiotics were administered by themselves (92%). A total of 280 samples (40 each
from piglets, weaners and adults, 20 feed, 30 water and 20 hand swabs from 10 pig farms
and 20 each from meat samples, cutting board, hand swabs and knives swab and 10 water
sample from retail pork outlets ) were screened and 227 E. coli isolates were used for
further characterization. The average E. coli count ranged from 6.104 to 6.464 log10cfu/g.
The overall occurrence of E. coli from various samples from pig production system was
67.89 per cent (129/190). The highest per cent of AMR E. coli counts as proportion of
total E. coli was observed in human hand swabs (83.90%) followed by water trough
(30.56%) and piglets (22.06%) samples as compared to other samples in this study. The
overall prevalence of tetracycline, fluoroquinolone, ESBL resistant and colistin resistant
E. coli in the entire pork production chain irrespective of the samples was 52.11, 37.37,
17.89 and 13.16 per cent, respectively. Of the 208 isolates, 191 isolates (91.83%) carried
either one or the other gene, whereas, only 8.17 per cent of the isolates did not harbor any
of the genes screened. Majority of the isolates carried tetA (68.38%), followed by
blaTEM
(55.77%), qnrS (44.23%), and none of the isolates carried colistin resistance gene
(mcr1 to mcr5). Antimicrobial susceptibility testing revealed that majority of the isolates
showed resistance to tetracycline (86.96-100%) followed by extended-spectrum b
lactamase inhibitors and fluoroquinolones and complete sensitivity to carbapenem
followed by aminoglycosides. It was observed that 96.92 per cent of E. coli isolates were
MDR. The occurrence of ESBL and colistin resistant E. coli was 17.89 and 0.714 per
cent per cent, respectively.