Isolation and antifungal evaluation of bioactive compounds from Vinca rosea
| dc.contributor.advisor | Ramandeep Kaur | |
| dc.contributor.author | Sharma, Parul | |
| dc.date.accessioned | 2022-07-30T14:04:56Z | |
| dc.date.available | 2022-07-30T14:04:56Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Vinca rosea is an evergreen plant belonging to family Apocynaceae possessing numerous medicinal properties. The present work deals with the extraction and chemical investigation of V. rosea leaves and flowers extracts, leaves essential oil and its potential as fungicide against two phytopathogenic wheat fungi i.e., Fusarium graminearum and Bipolaris sorokiniana. Leaves and flowers extracts were prepared using six different solvents. GC-MS analysis of the methanolic extract of leaves revealed tetradec-7-enal (19.61%), glycerol (17.58%) and palmitic acid (16.38%) as the major compounds whereas methanolic extract of flowers showed inositol (42.66%) as the major compound. Quantitative data revealed the presence of alkaloid in higher quantity in leaves. Vindoline, was isolated from the leaves of the plant and other major compounds were isolated by column chromatography from methanolic leaves and flowers extracts. Structures of isolated compounds were elucidated by FT-IR, 1HNMR, 13CNMR and Mass spectroscopic techniques. Fungitoxic activity of V. rosea leaves and flowers extracts, essential oil and isolated compounds was investigated by poisoned food technique at different concentrations (125-1000 µg/ml). Propiconazole (Tilt 25 EC) served as positive control. Methanol extract of leaves and flowers was found to be more effective in comparison to other extract. Among all the tested components, vindoline was observed to be the most active compound against F. graminearum (ED50 = 68 µg/ml) and B. sorokiniana (ED50 = 97 µg/ml). Further the activity was increased with increasing concentration but still less than the synthetic fungicides. | en_US |
| dc.identifier.citation | Sharma, Parul (2021). Isolation and antifungal evaluation of bioactive compounds from Vinca rosea (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810185939 | |
| dc.keywords | Antifungal activity, essential oil, extracts Vinca rosea and vindoline. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 151 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Isolation and antifungal evaluation of bioactive compounds from Vinca rosea | en_US |
| dc.sub | Chemistry | en_US |
| dc.theme | Isolation and antifungal evaluation of bioactive compounds from Vinca rosea | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Isolation and antifungal evaluation of bioactive compounds from Vinca rosea | en_US |
| dc.type | Thesis | en_US |