Isolation and antifungal evaluation of bioactive compounds from Vinca rosea
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Vinca rosea is an evergreen plant belonging to family Apocynaceae possessing numerous
medicinal properties. The present work deals with the extraction and chemical investigation
of V. rosea leaves and flowers extracts, leaves essential oil and its potential as fungicide
against two phytopathogenic wheat fungi i.e., Fusarium graminearum and Bipolaris
sorokiniana. Leaves and flowers extracts were prepared using six different solvents. GC-MS
analysis of the methanolic extract of leaves revealed tetradec-7-enal (19.61%), glycerol
(17.58%) and palmitic acid (16.38%) as the major compounds whereas methanolic extract of
flowers showed inositol (42.66%) as the major compound. Quantitative data revealed the
presence of alkaloid in higher quantity in leaves. Vindoline, was isolated from the leaves of
the plant and other major compounds were isolated by column chromatography from
methanolic leaves and flowers extracts. Structures of isolated compounds were elucidated by
FT-IR, 1HNMR, 13CNMR and Mass spectroscopic techniques. Fungitoxic activity of V. rosea
leaves and flowers extracts, essential oil and isolated compounds was investigated by
poisoned food technique at different concentrations (125-1000 µg/ml). Propiconazole (Tilt 25
EC) served as positive control. Methanol extract of leaves and flowers was found to be more
effective in comparison to other extract. Among all the tested components, vindoline was
observed to be the most active compound against F. graminearum (ED50 = 68 µg/ml) and B.
sorokiniana (ED50 = 97 µg/ml). Further the activity was increased with increasing
concentration but still less than the synthetic fungicides.
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Citation
Sharma, Parul (2021). Isolation and antifungal evaluation of bioactive compounds from Vinca rosea (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.