Quantification of Mycobacterium avium subsp. paratuberculosis from the tissues of challenged mice using SYBR Green real time PCR assay for the assessment of vaccine efficacy

Simple item page
dc.contributor.authorChaitanya, RK
dc.contributor.authorKrishnamohan Reddy, Y
dc.contributor.authorDhinakar Raj, G, et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-02-27T07:27:23Z
dc.date.available2020-02-27T07:27:23Z
dc.date.issued2019-07
dc.descriptionTNV_IJAR_2019_53(7)944-948en_US
dc.description.abstractA SYBR Green real time PCR assay amplifying F57 gene of Mycobacterium avium subsp. paratuberculosis (MAP) was developed to evaluate the protective efficacy of the inactivated vaccine to prevent the colonization of MAP in the tissues of BALB/c mice challenge model. Bacterial burden in the liver, spleen and intestine of vaccinated and sham immunized control mice, after intra peritoneal challenge with MAP were quantified by real time PCR assay. A 195 bp fragment of MAP F57 sequence was cloned into TA cloning vector and the resultant recombinant plasmid was used to generate a series of quantification standards with copy number ranging from 109 to 1 copy. This absolute quantification technique is rapid and able to detect as few as 10 MAP organisms per gram of tissue and the assay has the efficiency of 0.982.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810143759
dc.keywordsHeat killed JD vaccine, Immunization of BALB/c mice and challenge, Mycobacterium avium subsp. paratuberculosis, MAP quantification, qRT-PCR assay.en_US
dc.language.isoenen_US
dc.pages944-948en_US
dc.relation.ispartofseries;7
dc.subjectVeterinary Scienceen_US
dc.titleQuantification of Mycobacterium avium subsp. paratuberculosis from the tissues of challenged mice using SYBR Green real time PCR assay for the assessment of vaccine efficacyen_US
dc.title.alternativeIndian Journal of Animal Researchen_US
dc.typeArticleen_US
dc.volume53en_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
TNV_IJAR_2019_53(7)944-948.pdf
Size:
271.85 KB
Format:
Adobe Portable Document Format
Description:
TNV_IJAR_2019_53(7)944-948
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Articles (1.Journal)