MOLECULAR CHARACTERIZATION OF A Tospovirus INFECTING BELL PEPPER (Capsicum annuum L.)

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dc.contributor.advisorHANDA, ANIL
dc.contributor.authorBOHRA, YOGITA
dc.date.accessioned2017-05-11T12:38:46Z
dc.date.available2017-05-11T12:38:46Z
dc.date.issued2015
dc.description.abstractABSTRACT Surveys conducted during the present investigations revealed the presence of ringspot disease in bell pepper growing areas of Solan district of Himachal Pradesh with disease incidence ranging between 5 percent to 51percent during the cropping seasons of 2013 and 2014. The causal virus was characterized on the basis of symptomatology, transmission, host range, serology and molecular studies. Infected bell pepper plants exhibited ringspot symptoms of variable sizes on the leaves which coalesced later on covering the entire leaf lamina and in some cases rings turning necrotic were also observed. Infected bell pepper fruits developed pale yellow, red and green ringspots. Symptomatic plants were collected and sap inoculated to healthy bell pepper plants. Samples from Khaltoo and Pandah yielded strong positive reaction when subjected to DAS-ELISA against Tospo (I, II, III) antisera. Transmission studies revealed that virus isolates were transmissible through sap but not through seed. Studies on indicator hosts established Nicotiana tabaccum var. White Burley and Nicotiana glutinosa to be the best indicator hosts for maintaining virus cultures round the year. Studies on the host range revealed that out of six families tested namely Chenopodiaceae, Cruciferae, Cucurbitaceae, Leguminosae, Malvaceae and Solanaceae, only members of family Cruciferae and Malvaceaeremained uninfected on sap inoculation however, other hosts variably produced localized and systemic symptoms. Serological assay of weed hosts revealed that Amaranthus sp. and Datura sp. to be the possible reservoir hosts of the causal virus. Infected tomato fruits growing in the vicinityof infected bell pepper fields however, yielded negative results in DAS-ELISA but RT-PCR based detection revealed clear association of causal virus with tomato. RT-PCR based detection, nucleocapsid (N) gene sequencing and BLASTN analysis of bell pepper virus isolates revealed that the virus associated with ringspot disease of bell pepper in Himachal Pradesh was Capsicum chlorosis virus, a member of genus Tospovirus.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810010987
dc.keywordsbell pepper , ringspot disease, Solan district of Himachal Pradeshen_US
dc.language.isoenen_US
dc.publisherUHF,NAUNIen_US
dc.subPlant Pathologyen_US
dc.subjectnullen_US
dc.themebell pepper , ringspot disease, Solan district of Himachal Pradeshen_US
dc.these.typeM.Scen_US
dc.titleMOLECULAR CHARACTERIZATION OF A Tospovirus INFECTING BELL PEPPER (Capsicum annuum L.)en_US
dc.typeThesisen_US
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