Cryopreservation studies in Callus culture of Arnebia euchroma

Simple item page
dc.contributor.advisorBhushan, Shashi
dc.contributor.authorRai, Sourav
dc.date.accessioned2022-05-10T09:31:25Z
dc.date.available2022-05-10T09:31:25Z
dc.date.issued2022-05-09
dc.description.abstractArnebia euchroma commonly known as Ratanjot belongs to Boraginaceae family. Arnebia species is known for rich source of shikonin and their derivatives have antibacterial, antifungal, anti-HIV, anti-inflammatory and several other medicinal properties. The genus Arnebia has been over exploited as a result of these characteristics, and it is now classified as a endangered species. These resources must be protected in order to be used on a large scale in the future, and this valuable treasure must be preserved. Callus was induced from the leaves with (75%) induction when cultured on MS medium augmented with IBA (1.02 mg/l) and BAP (2.25 mg/l) and multiplied on same medium. Leaf derived callus were used for the cryopreservation Therefore, a protocol is optimized for cryopreservation of callus using vitrification, encapsulation vitrification and desiccation technique. Callus was precultured on sucrose enriched medium containing 3% (w/v) and 13.7% (w/v) sucrose. Among all these techniques of cryopreservation highest survival rate (65%) was found in desiccation when precultured on 13.7% sucrose followed by 90 minutes desiccation time. Callus used for the cryopreservation was not revived after cryogenic treatment and necrosed after eight weeks of culture in encapsulation vitrification method. Hence, this method of cryopreservation was not found suitable for preserving the callus tissues of A. euchroma. Non cryopreserved callus showed the highest total Deoxyshikonin content i.e. 369.28 (µg/gm) and 301.31 (µg/gm) shikonin content was observed and Cryopreserved callus showed the highest Deoxyshikonin content i.e. 319.23 (µg/gm) and total shikonin content of 296 (µg/gm). In UPLC chromatography only deoxyshikonin content i.e. 65.05 (µg/gm) was observed in non cryopreserved callus and 39.07(µg/gm) content was found in cryopreserved callus.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810184522
dc.keywordsspecies,culture,cryopreservation.en_US
dc.language.isoEnglishen_US
dc.pages42en_US
dc.publisherpalampuren_US
dc.subAgricultural Biotechnologyen_US
dc.themeEstablishment of callus cultures from Arnebia euchroma,Protocol for cryopreservation of callus culture and their regenerationen_US
dc.these.typeM.Scen_US
dc.titleCryopreservation studies in Callus culture of Arnebia euchromaen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Sourav Rai Msc.Agr.Bio.pdf
Size:
1.31 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses