―Phenotypic and molecular characterization of HD 2967 x Kharchia 65 F5 and backcross generations for salt tolerance in wheat (Triticum aestivum L. em. Thell”
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Date
2019-08
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CCSHAU, Hisar
Abstract
Soil salinity is emerging environmental stress that reduces rate of germination, delays
emergence and affects almost all stages of growth and development of wheat plants which
ultimately leads to reduction in grain yield of crop.The present investigation focused on
phenotypic and molecular characterization of selected wheat (Triticum aestivum L. em.
Thell)genotypes of F5 and backcross (BC1F4, BC2F3, BC3F1) generations derived from HD
2967 X Kharicha 65 for salinity tolerance on the basis of presence of both Nax loci and their
agronomic performance at salt stress level 8 dS/m. A total of 92 plants confirmed the presence
of Nax1 locus at a band size of 210 bp consisting of 16 F5, 41 BC1F3, 9 BC2F2 and 27 BC3F1, a
total of 53 plants confirmed the presence of Nax2 locus at a band size of 225 bp comprising of 6
F5, 35 BC1F4, 8 BC2F3 and 4 BC3F1.Overall, plants comprising of 6 F5,26 BC1F4, and 4 BC3F1 of
HD 2967 x Kharchia 65were found to have both the Nax loci. On the basis of phenotypic and
genotypic variations, 56 high yielding plants were selected for genotyping using polymorphic
SSR markers. A total of 180 SSR primers were used to study the polymorphism between
parents HD 2967 and Kharchia 65, out of which 27 SSR primers showed polymorphism.
Polymorphism information content (PIC) value in the studied genotyped ranged from 0.191
(xgwm480) to 0.425 (wmc3) with an average value of 0.339. Cluster tree analysis using
NTSYS-pc software, F5 and backcross (BC1F4, BC2F3, BC3F1) generations showed that all the
selected plants were inclined toward recurrent parent HD 2967 representing higher similarity
with HD 2967. From above analysis, high grain yielding and salt tolerant plants were selected
such as plant number 259Q3c, 271Q3c (F5); 202P9b, 303P2b and 202P3b (BC1F4); 241R2c
and 243R1a (BC2F3); 25(3)1b and 24(1)1c (BC3F1) and these plants could be further
backcrossed with the parent HD 2967 to develop salt tolerant wheat lines.