Title : ASSESSMENT OF SILICON EFFECT IN RESPONSE TO CHARCOAL ROT IN SOYBEAN (Glycine max L.).

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dc.contributor.advisorAdvisor : JADHAV, Dr. P. V.
dc.contributor.authorAuthors : KHODE, VAIBHAVI.
dc.date.accessioned2022-02-26T13:35:05Z
dc.date.available2022-02-26T13:35:05Z
dc.date.issued2022-10-04
dc.descriptionDescription : Silicon is known to develop tolerance against fungal diseases in plants. The present study showed the deposition of silicon of 0.7 mM and 1.7 mM treatment in the trichomes of susceptible and resistant soybean genotypes. The genotypes showed enhanced morphological characters at phenotypic level and cDNA-RAPD profiling generated showed expression of charcoal rot disease tolerance at molecular level.en_US
dc.description.abstractAbstract : The present study entitled “Assessment of silicon effect in response to charcoal rot in soybean (Glycine max L.)” was carried out at Department of Agricultural Botany, Dr. PDKV, Akola during the academic year 2020-21 to evaluate the effect of silicon in response to charcoal rot disease in soybean genotypes. Considering the severity of the disease, potassium silicate (K2SiO3) which is known for activating defense mechanism and enhancing the morphological characters in plants. To identify effect of K2SiO3, mass culture of M. phaseolina was prepared for in-vitro screening under sick pot condition for charcoal rot tolerance in soybean genotypes. Silicon gets accumulated in higher concentration in the epidermal cells and has been detected to benefit the plants that are exposed to biotic stresses. The susceptible genotype, TAMS-38 in charcoal rot infected condition showed disease symptoms whereas resistant genotype AMS-MB-5-18 showed normal growth. But in both the treatments 0.7mM and 1.7mM of K2SiO3 (K2SiO3 + C.R. infected), TAMS-38, AMS-MB-5-18 and JS-335 showed tolerance to charcoal disease as well as healthy growth. To identify the amount of accumulation of silicon in the susceptible and resistant genotypes, SEM-EDX analysis was done. The result revealed higher deposition of the silicon in the trichomes of the susceptible genotype. Accumulation of Si in the genotypes and plant response against charcoal rot disease with different morphological observations was recorded. To assess the in-vitro effect of silicon at phenotypic level various morphological characters were recorded such as plant height (cm), number of branches, 50% flowering (D), days to maturity (D), number of pods per plant, grain yield per plant (g) and 100 seed weight (g). The analysis of variance showed significant difference between the genotypes of different concentrations of Si treated pots (0.7mM and 1.7mM of K2SiO3) with charcoal rot, control pot and Si control. The phenotypic screening of soybean genotype against charcoal rot disease revealed that K2SiO3 leads to better adaptability in soybean genotype under charcoal rot disease condition. The effect of Si was also studied at molecular level by using cDNA-RAPD profiling which revealed due to K2SiO3 treatments various genes were activated in response to charcoal rot disease. Each cluster represents different response towards charcoal rot in each individual genotype. Out of the total screened alleles 75 were monomorphic alleles with an average of 3.75 and 69 were polymorphic alleles with an average of 3.45. Results showed an average of 47.68 polymorphism percent. The IDVs (intensity derived values) were calculated to study expression of varying size of polymorphic amplicons. The highest IDV (241002) value was recorded for primer OPH-12 in 1.7 mM of K2SiO3 treated plant of susceptible genotype TAMS-38. The study revealed that both the treatments 0.7mM and 1.7mM of K2SiO3 showed healthier growth in all three soybean genotypes. 1.7mM of K2SiO3 treatment showed superior results at morphological as well as molecular level.en_US
dc.identifier.citationCitation : KHODE, VAIBHAVI. (2021). ASSESSMENT OF SILICON EFFECT IN RESPONSE TO CHARCOAL ROT IN SOYBEAN (Glycine max L.). Biotechnology Centre, Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2021. Print. xiii, 88p. (Unpublished)en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182959
dc.keywordsKeywords : Glycine max L., M. Phaseolina, Silicon, RNA, cDNA-RAPD Profiling, SEM-EDX analysis,en_US
dc.language.isoEnglishen_US
dc.pagesPages : xiii, 98p. (Unpublished)en_US
dc.publisherPublisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.en_US
dc.research.problemResearch problem : In the present study the researcher has tried to study uptake and accumulation of silicon in soybean genotypes under in-vitro condition and to study effects of silicon supplementation in response to charcoal rot in soybean and comparative molecular analysis of resistant and susceptible genotypes using molecular markers.en_US
dc.subAgricultural Biotechnologyen_US
dc.themeTheme : The present investigation aims to assessment of silicon effect in response to charcoal rot in soybean (glycine max L.).en_US
dc.these.typeM.Scen_US
dc.titleTitle : ASSESSMENT OF SILICON EFFECT IN RESPONSE TO CHARCOAL ROT IN SOYBEAN (Glycine max L.).en_US
dc.typeThesisen_US
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Description : Silicon is known to develop tolerance against fungal diseases in plants. The present study showed the deposition of silicon of 0.7 mM and 1.7 mM treatment in the trichomes of susceptible and resistant soybean genotypes. The genotypes showed enhanced morphological characters at phenotypic level and cDNA-RAPD profiling generated showed expression of charcoal rot disease tolerance at molecular level.
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