EXPLOITING THE BIO CONTROL POTENTIALITY OF Bacillus velezensis P42 AND A6 STRAINS AGAINST IMPORTANT BACTERIAL WILT AND EARLY BLIGHT DISEASES OF TOMATO
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Date
2018-08-01
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University of Agricultural Sciences, Bangalore
Abstract
Tomato is a prominent vegetable crop which is frequently affected by bacterial wilt and early blight demanding integration of biological features in its management. Whole genome sequencing of Bacillus velezensis strains P42 revealed genome size of 4.04 mb with 4028 protein-coding genes having 452 unique genes with 3576 conserved genes and 40 biosynthetic gene clusters. B. velezensis A6 and P42 were formulated into 14 solid and 10 liquid formulations, among them tablet (P42-log1010.54 and A6- log1010.50 cfu g-1) and 10mM glycerol formulation exhibited highest population (P42-
log1010.79 and A6-log10 9.61 cfu ml-1). Tablets exhibited inhibition zone of 3.41 cm (P42) and 3.3 cm (A6) whereas, nutrient broth P42 exhibited 1.00 cm and 1.37 cm (10mM glycerol A6) against R. solanacearum. Talc formulation of both strain resulted in 80.49 per cent mycelial inhibition followed by 10mM trehalose of A6 (65.85%) and P42
(63.41%) against A. solani. In vivo studies revealed that tablet, YE (1%) and tryptone
(1%) formulations of P42 exhibited 25 per cent wilt incidence while tablet formulation of
A6 showed 100 per cent control. Both A6 and P42 of 10mM glycerol, nutrient-broth,
poly vinyl pyrrolidine (2%), 10mM trehalose exhibited 25 per cent wilt incidence. P42 of
tablet formulation recorded 26.67 per cent and YE (1%) of A6 recorded 20.00 per cent
disease severity while 10mM glycerol and 10mM trehalose of P42 exhibited 26.67 per
cent and 10mM glycerol of A6 exhibited 20 per cent disease severity against A. solani.
The two strains were compatible with important fungicides and antibiotics.