ANTIOXIDANT PROPERTIES AND MOLECULAR DIVERSITY OF MEETHI NEEM (MURRAYA KOENIGII (L.) SPRENG)
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Date
2018-02-09
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CSKHPKV, Palampur
Abstract
Fifteen wild Meethi Neem leaf samples collected at three stages viz. pre-flowering, flowering and fruiting stages from different locations of Kangra and Mandi district of Himachal Pradesh were analyzed to evaluate phytochemical constituents and to identify the genetic diversity. The range of variation for phytochemical constituents at three stages varied significantly viz. total chlorophyll (0.79 to 1.43, 1.31 to 1.94 and 0.88 to 1.10 mg/g), total carotenoids (30.55 to 49.25, 49.09 to 60.81 and 31.24 to 41.42 μg/g), ascorbic acid (7.01 to 9.82, 3.58 to 6.06 and 2.99 to 4.93 mg/100g), total phenol (170 to 304, 266 to 450 and 213 to 364 mg/g), simple phenol (93 to 163, 150 to 243 and 123 to 220 mg/g), tannins (76 to 143, 116 to 207 and 47 to 156 mg/g), flavonoids (15.17 to 33.40, 25.16 to 58.17 and 17.54 to 37.34 mg/g), polyphenol oxidase (0.030 to 0.058, 0.017 to 0.031 and 0.007 to 0.021 ΔOD/minute), antioxidant activity (4.01 to 7.42, 8.08 to 13.60 and 3.11 to 6.37 μg/ml), essential oil (0.64 to 0.89, 0.85 to 1.00 and 0.54 to 0.70 per cent) and quinones (2.05 to 2.97, 3.07 to 4.95 and 1.02 to 1.96 mM/min/g tissue). All parameters showed highest value at flowering stage except ascorbic acid and polyphenol oxidase activity. Clustering analysis with respect to biochemical constituents revealed that the geographically distinct collections of Meethi Neem were clustered together. For molecular characterization by ISSR, a total of 120 highly reproducible bands were obtained out of which 74 were polymorphic. The polymorphism ranged from 12.5 to 100 per cent. Dendrogram generated using UPGMA separated 10 collections into 2 major clusters with 46 to 100 per cent similarity. The overall analysis revealed that Meethi Neem collections from the same geographical location were genetically and biochemically diverse.
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