Hybridization potential of Chinese spring wheat with indigenous cultivated barley and haploid induction efficiency of their derivatives following chromosome elimination- mediated systems
Loading...
Date
2021-11-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Palampur
Abstract
The research endeavour entitled “Hybridization potential of Chinese spring wheat with
indigenous cultivated barley and haploid induction efficiency of their derivatives following
chromosome elimination- mediated systems” was executed in the Department of Genetics and Plant
Breeding, CSK HPKV, Palampur during the years 2019-20 and 2020-21 with the goal to work out
relative efficiency of Chinese spring wheat × cultivated indigenous barley and induce haploids from the
BC1F1 generation of Chinese spring wheat × cultivated indigenous barley through Imperata cylindrica
and maize- mediated chromosome elimination technique of doubled haploidy breeding. The material
for present investigation comprised of two bread wheat genotypes; Chinese spring wheat and C306, ten
potential barley landraces collected from north- west Himalayan regions and their F1s & backcross
derivatives. During rabi 2019-20, hybridization work was carried out using wheat genotypes as female
parent and barley genotypes as male parent followed by estimating the frequency of seed setting
induced by the barley genotypes in wheat parental lines. The first filial generation produced after
hybridization was sown at HAREC, Kukumseri to deduce the frequency of seed setting for the
formation of backcross generation. Chinese spring wheat genotype performed better than C306 for
development of F1s while Barley 3 and Barley 10 reported significantly higher response for
hybridization with Chinese spring wheat and C306, respectively. The mean seed setting frequency
recorded for the development of F1 and BC1F1 generation was 0.76% and 16.34% in Chinese spring
wheat while 0.37% and 20.04% in C306, respectively. For all the parameters of haploid induction viz.,
pseudoseed formation, embryo formation and regeneration, significant differences were recorded in all
the wheat/ barley derivatives depicting genotype specificity for haploid induction. Based on statistical
analysis, it was revealed that relative efficiency of I. cylindrica was higher than maize for haploid
induction. C306 performed better than Chinese spring for induction on haploids following I. cylindrica
mediated- chromosome elimination technique. Bread wheat genotypes performed better than the
backcrosses following F1 and F2 genotypes towards haploid induction parameters. Barley 4 and Barley
9 performed better for hybridization and haploid induction in Chinese spring wheat and C306,
respectively than other barley landraces. This attempt of hybridizing wheat with barley; producing
haploids from their backcrosses especially BC1F1s, which can be transformed into double haploids by
colchicine treatment has opened the dimensions with enhanced opportunities of genetic upgradation of
wheat utilizing gene(s) from barley