ISOLATION AND MOLECULAR CHARACTERIZATION OF INDIGENOUS GOATPOX VIRUS FROM TELANGANA REGION
| dc.contributor.advisor | SATYANARAYANA CHETTY, M(MAJOR) | |
| dc.contributor.advisor | Anjaneyulu, Y | |
| dc.contributor.author | VIJAYALAXMI, V | |
| dc.date.accessioned | 2018-10-25T09:36:51Z | |
| dc.date.available | 2018-10-25T09:36:51Z | |
| dc.date.issued | 2009-01 | |
| dc.description | THESES | en_US |
| dc.description.abstract | ABSTRACT:In view of the increasing incidence of goatpox out breaks the present study has been taken up with a view to isolate goatpox virus from field cases and characterize them. The disease surveillance reports on goatpox were collected for the years 1997-2008 and analysed. The analysis of epidemiological data revealed 176 outbreaks in Andhra Pradesh The outbreaks were recorded more during the years 2000-2003 and 2004-2007.Case fatality rates ranged from 3.7% - 49.4 % during the period. Samples were collected from two outbreaks of goatpox. Both the samples were positive for goatpox by Agar gel immunodiffusion. Attempts were made to isolate the virus by passaging in chicken embryos and then adapted to vero cell lines. Infected embryonating chicken eggs died between 48 h. and 120 h. Post Inoculation (PI) depending on the passage. CAM was edematous, thickened and congested. Slight hemorrhages were observed in 3rd passage. Embryo material after 3rd passage gave positive reaction when tested by Agar gel immunodiffusion with positive serum. EID50/ml (embryo infective dose 50) of the virus isolates ranged from 0.5x102 to 1x102 for the virus from 1st isolate while the virus from 2nd isolate had titres ranging from 0.7x102 to 0.9x102 / ml. Embryo passaged virus was then adapted to vero cells. On first passage cells revealed granulation and shrinkage of cells at 24 h – 48h post infection, syncitia at 72 h - 96 h PI while peeling of monolayer started at 120 h PI. Complete detachment of monolayer was seen in 6 days. Virus from 1st isolate had a titre of 104.9 TCID50 /ml and 2nd isolate had 105.2 TCID50/ml titre. Goatpox virus (GPV) was successfully purified by sucrose density gradient centrifugation. Two bands formed on the gradient, one at the interface of 50 and 60 percent sucrose layer and another at 40 percent sucrose layer. AGID and Immuno Electrophoresis were used to characterize the isolates. They resulted in a single precipitation line with hyperimmune serum. Physicochemical characterization of the viruses with regard to temperature, pH, ether and chloroform were carried out. The viruses were completely inactivated at 60oC in 30 min and 70oC in 10 min. Chloroform, ether and pH 3 were detrimental to goatpox viral isolates. Electron microscopy of the virus isolates revealed brick shaped virus with size of 250 x 290 nm. SDS - Polyacrylamide gel electrophoresis of the isolates revealed a total of 20 bands. Of these, five were major bands with size ranging from 22 to 67kd. Seroprevalance studies on goatpox was studied by employing AGID and Counter Immuno Electrophoresis (CIE). CIE was found more sensitive with positive percentage of 22.5 compared to 13.75 by AGID in a total of 80 serum samples. Polymerase chain reaction was used for detection of specific nucleotide sequences of GPV in samples and cell culture adapted viruses | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810082125 | |
| dc.keywords | ISOLATION;MOLECULAR CHARACTERIZATION;INDIGENOUS GOATPOX VIRUS;TELANGANA REGION | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 127 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | ISOLATION AND MOLECULAR CHARACTERIZATION OF INDIGENOUS GOATPOX VIRUS FROM TELANGANA REGION | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | ISOLATION AND MOLECULAR CHARACTERIZATION OF INDIGENOUS GOATPOX VIRUS FROM TELANGANA REGION | en_US |
| dc.type | Thesis | en_US |