Clinico-pathological and therapeutic studies on colibacillosis in neonatal calves

dc.contributor.advisorA., Janus
dc.contributor.authorMATHEW, XAVIER
dc.date.accessioned2021-01-12T07:32:56Z
dc.date.available2021-01-12T07:32:56Z
dc.date.issued2019-08-19
dc.description.abstractColibacillosis is an economically important disease, causing neonatal diarrhoea and septicaemia in calves. It has worldwide occurrence and has been reported from almost all states of India. The present study was conducted to identify different strains of Escherichia coli associated with colibacillosis in calves from Wayanad district by identification of virulence factors through PCR, assessment of clinical and haemato-biochemical changes associated with colibacillosis and to suggest a suitable treatment protocol for neonatal colibacillosis. Virulence factors of pathogenic E. coli were targeted using specific primers against K99, F41, STa, intimin, Stx1 and Stx2 genes. Seventeen samples were found positive for Intimin, Stx1 and Stx2 together or alone suggesting the presence of EHEC/ EPEC. One milk sample and one drinking water sample were found positive for intimin and Stx1 which confirmed EHEC in the sample. A multiscreen Ag ELISA kit was used for ruling out bovine Rotavirus, Coronavirus, E coli F15 and Cryptosporidium antigens but it showed negative results for all the pathogens. Haemato-biochemical values and clinical parameters of all the thirty calves when compared with control, showed significant difference and all the abnormal parameters came to normal range after treatment. Sulphamethoxazole/ trimethoprim gave sensitivity to 18 isolates which was similar to clinical response, that is usage of sulphadiazine -trimethoprim showed fast recovery in 18 cases. In six cases ceftriaxone was used five cases amoxicillin antibiotic was used as per antibiogram and both the antibiotics showed good response. Calves were treated with balanced electrolyte solutions and sodium bicarbonate to correct metabolic acidosis, dehydration and electrolyte imbalances administration was very helpful to control metabolic acidosis. Detailed study to identify the diarrhoeagenic E coli strains is necessary as E coli could affect livestock farming and even, they can enter food chain tooen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810159890
dc.language.isoEnglishen_US
dc.pages93en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemstudies on colibacillosis in neonatal calvesen_US
dc.subVeterinary Epidemiology and Preventive Medicineen_US
dc.themeClinico-pathological and therapeutic studies on colibacillosis in neonatal calvesen_US
dc.these.typeM.V.Sc.en_US
dc.titleClinico-pathological and therapeutic studies on colibacillosis in neonatal calvesen_US
dc.typeThesisen_US
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