Clinico-pathological and therapeutic studies on colibacillosis in neonatal calves
Loading...
Date
2019-08-19
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
Colibacillosis is an economically important disease, causing neonatal
diarrhoea and septicaemia in calves. It has worldwide occurrence and has been
reported from almost all states of India. The present study was conducted to identify
different strains of Escherichia coli associated with colibacillosis in calves from
Wayanad district by identification of virulence factors through PCR, assessment of
clinical and haemato-biochemical changes associated with colibacillosis and to
suggest a suitable treatment protocol for neonatal colibacillosis.
Virulence factors of pathogenic E. coli were targeted using specific primers
against K99, F41, STa, intimin, Stx1 and Stx2 genes. Seventeen samples were found
positive for Intimin, Stx1 and Stx2 together or alone suggesting the presence of
EHEC/ EPEC. One milk sample and one drinking water sample were found positive
for intimin and Stx1 which confirmed EHEC in the sample.
A multiscreen Ag ELISA kit was used for ruling out bovine Rotavirus,
Coronavirus, E coli F15 and Cryptosporidium antigens but it showed negative results
for all the pathogens.
Haemato-biochemical values and clinical parameters of all the thirty calves
when compared with control, showed significant difference and all the abnormal
parameters came to normal range after treatment.
Sulphamethoxazole/ trimethoprim gave sensitivity to 18 isolates which was
similar to clinical response, that is usage of sulphadiazine -trimethoprim showed fast
recovery in 18 cases. In six cases ceftriaxone was used five cases amoxicillin
antibiotic was used as per antibiogram and both the antibiotics showed good
response. Calves were treated with balanced electrolyte solutions and sodium
bicarbonate to correct metabolic acidosis, dehydration and electrolyte imbalances
administration was very helpful to control metabolic acidosis.
Detailed study to identify the diarrhoeagenic E coli strains is necessary as E coli
could affect livestock farming and even, they can enter food chain too