STUDIES ON SUBCLINICAL KETOSIS IN BUFFALOES

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Date
2020-06
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA
Abstract
The present study was carried out to assess the prevalence of subclinical ketosis among 900 buffaloes from organized and unorganized herds, out of which 179 buffaloes were screened. The prevalence of subclinical ketosis was 20.67 % (37/179) based on serum BHB values. The high prevalence was observed in early lactation and among buffaloes of third lactation. Majority of the affected buffaloes (70.27 %) were high yielders with >10 lts of milk/day. The diagnostic efficacy of modified Rothera’s test and hand held ketometer were compared with serum BHB estimation by laboratory method. The blood BHB levels using hand held meter had significant correlation (Pearson’s r=0.965; p<0.01) with the serum BHB levels measured by the colorimetry. The sensitivity and specificity of modified Rothera’s test was 21.62% and 100% while that of hand held meter was 95% and 90%, respectively. The results of the study showed that serum glucose, serum cholesterol, triglycerides, TAC, calcium and phosphorus concentrations were significantly lower (P<0.05) in buffaloes with subclinical ketosis. In addition, there was positive correlation between the serum BHB and MDA and negative correlation between BHB and glucose, cholesterol, triglycerides, total proteins, calcium, phosphorous and zinc. A total of 16 buffaloes were randomly divided into two groups (I and II). Group I buffaloes were treated with conventional therapy Gluca-boost® @ 200 ml/day for 6 days while Group II were treated with SEZ® @ 20 gms/day for 20 days along with Gluca-boost®. The therapeutic evaluation was done based upon clinical improvement, alterations in serum biochemical parameters, MDA and TAC. The maximum improvement was noticed in Group II buffaloes with more prominent reduction in MDA and improvement in TAC was in this group. The comparison of pre-therapeutic values of BHB, glucose, serum cholesterol, MDA and TAC revealed significant improvement in Group II compared to Group I. The therapeutic outcome was better with incorporation of trace elements vitamin E, selenium and zinc with antioxidant properties.
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