DNA MARKER-ASSISTED IDENTIFICATION OF GENOMIC REGIONS CONTROLLING ANTHESIS-SILKING
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Date
2016-12-27
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
An investigation was undertaken to identify QTLs/SSR markers linked to genomic
regions controlling Anthesis-Silking Interval (ASI) and grain yield and to unravel the
genetics of ASI, grain yield and its components using generation mean analysis and
Triple Test Cross analysis from summer 2013 to rabi 2014 at Department of Genetics and
Plant Breeding, University of Agricultural Sciences, GKVK, Bengaluru. Two
polymorphic inbred lines MAI-349 and BGD-89 were involved to generate F2 mapping
population and this F2 population was genotyped with 40 SSR markers which had
discriminated the inbred lines MAI-349 and BGD-89. The corresponding F2:3 progeny
families were evaluated for ASI, grain yield and its component traits. Single marker
analysis revealed association of eleven SSR markers controlling ASI which had
explained phenotypic variance ranging from 5.41 to 12.50% and nine markers linked with
grain yield plant-1 and had explained phenotypic variance ranging from 5.99 to 8.20%.
Two QTLs linked to ASI detected on chromosome 5 and 7 showed dominant and overdominant
gene actions, respectively, and were flanked by umc1523-umc1829 with
phenotypic variance of 16.25% and umc2334-umc1792 with phenotypic variance of
5.51%, respectively. Three minor QTLs controlling grain yield were detected on
chromosomes 6 (one QTL with phenotypic variance 4.29%) and 9 (two QTLs with
phenotypic variance 7.71 and 6.73 %, respectively) displayed over-dominance and were
flanked by the markers umc1014-umc1020, umc1387-phi065 and phi065-umc1714.
Epistasis played a significant role in the inheritance of all the characters studied.
Preponderance of additive component for all the traits indicated the utility of
improvement through simple selection. Reciprocal recurrent selection can be an effective
breeding method for the improvement of those characters controlled by dominant gene
action
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