DISTRIBUTION AND BIOCONTROL POTENTIAL OF ENTOMOPATHOGENIC NEMATODES AGAINST SOME LEPIDOPTEROUS PESTS

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dc.contributor.advisorChandel, Y. S.
dc.contributor.authorVASHISTH, SUMIT
dc.date.accessioned2016-08-03T15:36:10Z
dc.date.available2016-08-03T15:36:10Z
dc.date.issued2014
dc.descriptionDoctoral Dissertationen_US
dc.description.abstractSurvey for entomopathogenic nematodes (EPNs) was conducted in ten districts of Himachal Pradesh during 2011-13. In all, 930 soil samples from 131 locations were analysed for the occurrence of the nematodes. The frequency of occurrence of these nematodes was very low (3.82%). The nematodes were found in five locations i.e. Sangla (Distt. Kinnaur), Kamand (Distt. Kullu), Rajgarh (Distt Sirmour), Rajour and Sutkar (Distt. Chamba). These localities are situated in mid hill/high hill zones of the state. The nematodes were recovered from fruit orchards only having lighter soils. The nematodes from all the locations were identified as Heterorhabditis. Nematode cultures from different locations were considered as isolates and were designated as ‘Sangla isolate, ‘Kamand isolate’, ‘Rajgarh isolate’, ‘Sutkar isolate’ and ‘Rajour isolate’. Former three isolates, along with one commercial isolate i.e. H. indica (procured from NBAII, Bangluru) were evaluated for their pathogenic effects against Spodoptera litura, Helicoverpa armigera, Plutella xylostella and Agrotis segetum in Petri plates/soil/screen house. All these three local isolates including the commercial isolate proved pathogenic variably to different insect-pests. In screen house testing on cabbage, ‘Rajgarh isolate’ was found highly effective against S. litura, H. armigera and P. xylostella. The virulence of these isolates was considered of the medium level. The bacterium, Photorhabdus luminescence was associated with all the isolates and proved non pathogenic to Achroia grisella when tested alone. Besides, host invasion studies in A. grisella revealed maximum penetration through natural openings, other than mouth. For in vivo mass production of local EPNs, Corcyra cephalonica, A. grisella and Bombyx mori were used. The average yield of IJs/larva was minimum (23020-26030) in C. Cephalonica and maximum in B. mori (463080-546840) for different EPNs. For in vitro production, chicken offal medium was used. The yield was maximum for ‘Rajgarh isolate’ (17.70 x 106 IJs/flask).en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/70762
dc.language.isoenen_US
dc.publisherCSK Himachal Pradesh Krishi Vishavavidyalaya, Palampuren_US
dc.subEntomology
dc.subject##Unable to generate tags sumit vashisth phd 2014.pdfen_US
dc.subjectLEPIDOPTEROUS PESTSen_US
dc.subject##Unable to generate tags sumit vashisth phd 2014.pdfen_US
dc.subjectInsectsen_US
dc.these.typePh.D
dc.titleDISTRIBUTION AND BIOCONTROL POTENTIAL OF ENTOMOPATHOGENIC NEMATODES AGAINST SOME LEPIDOPTEROUS PESTSen_US
dc.typeThesisen_US
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