Assessment of Genetic Variability in Murrah Buffaloes through DNA Fingerprinting
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Date
2013-11
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT :
The present study was undertaken for assessment of genetic variability in Murrah buffaloes through DNA fingerprinting using a total of 30 microsatellite markers selected from the list suggested by FAO. The mean optical absorbance ratio (260/280nm) was 1.664 indicating good quality of genomic DNA and the mean quantity of DNA was 1.281μg/μl. All the 30 microsatellites were successfully amplified by polymerase chain reaction and observed number of alleles ranged from 4 (CSSM032 and ILSTS005) to 12 (CSSM033 and ILSTS028) with a total of 239 alleles across the 30 loci. The overall mean observed heterozygosity, expected heterozygosity and unbiased expected heterozygosity values were 0.439, 0.826 and 0.841 and ranged from 0.000 to 0.967; 0.709 to 0.899; and 0.754 to 0.915, respectively. The overall mean PIC was 0.803. All the 30 primers used in the present study were found to be polymorphic and highly informative with the PIC values ranging from 0.656 to 0.879. The overall mean inbreeding coefficient (FIS) and the outcrossing rate obtained in the present study were 0.483 and 0.464, respectively. Out of 30 microsatellite marker primers studied, 29 markers showed significant deviation from Hardy - Weinberg equilibrium which might be because of heterozygote deficiency. The sufficiently high mean values of observed number of alleles, observed heterozygosity and PIC for various microsatellites in the present study supported their
suitability for genetic diversity studies. Results of the present study have contributed to the knowledge of genetic information of Murrah buffaloes.