UNDERSTANDING THE ROLE OF VIRUS INDUCED TERPENOID SYNTHESIS REGULATION IN VIRUS-HOST-VECTOR INTERACTIONS
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Date
2018-08-01
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University of Agricultural Sciences, Bangalore
Abstract
Understanding the virus-host-vector relation is key to design management strategies against viral diseases of crop plants and attempt was made to understand the same in the current study. The Tomato leaf curl virus (ToLCV) was transmitted by whitefly Bemisia tabaci to tomato (Arka Vikas) plants, which produced leaf curl symptoms. TMV, ChiVMV and CMV biologically purified on Nicotiana glutinosa, Datura metel and cucumber plants, respectively and sap inoculated to tomato, chilli (Kashi Anmol) and N. glutinosa, respectively which produced characteristic symptoms. Molecular detection of TMV, ChiVMV and CMV by RT-PCR using CP gene specific primers produced an expected amplicon of 800 bp in all cases and ToLCV by PCR using begomovirus specific primers resulted in expected amplicon of 1400 bp. In Y- tube choice assay for ToLCV and TMV infected tomato plants, more response of non viruliferous whiteflies towards infected plants and ToLCV viruliferous whiteflies towards healthy plants was observed. There was no difference in response towards ChiVMV infected and healthy chilli plants. However,
difference was observed in case of ChiVMV infected D. metel plants and CMV infected N. glutinosa with more towards healthy plants. Differential gene expression analysis through Real time-PCR for four genes involved in terpenoid synthesis in ToLCV infected tomato leaf samples, showed the down regulation of DXS, ISPD and Pins genes and up regulation of HMGR gene. In TMV infected tomato leaf samples, DXS and ISPD genes were down regulated, HMGR gene was up-regulated and there was no differential regulation of Pins gene.