CHARACTERIZATION, EPIDEMIOLOGY, HOST PLANT RESISTANCE AND MANAGEMENT OF URDBEAN LEAF CRINKLE VIRUS (ULCV) IN BLACKGRAM (Vigna mungo L. Hepper)
| dc.contributor.advisor | Ramappa, H. K. | |
| dc.contributor.author | AISHWARYA, P. | |
| dc.date.accessioned | 2023-01-05T02:12:58Z | |
| dc.date.available | 2023-01-05T02:12:58Z | |
| dc.date.issued | 2022-02-17 | |
| dc.description.abstract | Urdbean Leaf Crinkle Virus (ULCV) is a major threatening disease in blackgram. The biological characterisation of ULCV revealed the sap transmission between 92-100 per cent, seed transmission between 55-95 per cent and Aphis craccivora C.L. Koch transmission upto 100 per cent with 10 minute of Acquisition Feeding Period, 12 and 24 hours of Inoculation Feeding Period with 10 aphids per plant. The host range studies revealed that soybean, field bean, horsegram, cowpea, greengram and weed Euphorbia geniculata to be hosts for ULCV disease. The rate of infection was severe at younger stages upto 35 DAS and reduced at later growth stages upon sap inoculation. Transmission Electron Microscopic observations of ULCV infected blackgram indicated isometric particles with size of 25-30 nm. The ULCV samples of blackgram showed no amplification for any of the primers of potyvirus, geminivirus and viroids. Metagenomic sequencing of ULCV samples showed nucleotide and amino acid identity of 76.44 and 90.66 per cent with Cotesia glomerata bracovirus (putative histone 4 gene) respectively which can be validated further. None of the 124 blackgram genotypes screened under controlled conditions showed resistant reaction for ULCV disease. The minimum (16- 180C) and maximum (32-340 C) temperature range co-related negatively and positively during late Kharif and Summer for ULCV disease incidence respectively. Seed treatment of thiamethoxam 30 % FS @ 10 ml-kg of seeds with neem (oil based) spray 10,000ppm @ 2 ml-L of water at 30, 45 and 60 DAS reduced the ULCV disease incidence compared to control. | en_US |
| dc.identifier.other | Th-13147 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810191221 | |
| dc.keywords | PLANT RESISTANCE, URDBEAN LEAF, CRINKLE VIRUS, BLACKGRAM | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 182 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | URDBEAN LEAF CRINKLE VIRUS (ULCV) IN BLACKGRAM | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | CHARACTERIZATION, EPIDEMIOLOGY, HOST PLANT RESISTANCE AND MANAGEMENT OF URDBEAN LEAF CRINKLE VIRUS (ULCV) IN BLACKGRAM (Vigna mungo L. Hepper) | en_US |
| dc.type | Thesis | en_US |