STUDIES ON MANAGEMENT OF CANINE MAMMARY TUMOURS WITH DENDRITIC CELL THERAPY

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dc.contributor.authorVALA ARSHI KALUBHAI
dc.contributor.editorDr. P. V. PARIKH
dc.date.accessioned2018-01-16T08:55:03Z
dc.date.available2018-01-16T08:55:03Z
dc.date.issued2016
dc.description.abstractThe present study was carried out with multiple objectives to standardize the treatment protocol for dendritic cell therapy for mammary gland tumours, to study the safety and tolerability of dendritic cell based therapy for the treatment of mammary gland tumour in dogs, and to evaluation of efficacy of dendritic cell therapy in dogs. Twenty three cases of canine mammary tumours were studied in detail. The highest occurrence of canine mammary tumour cases was found in the dogs aged 8 to 12 years (47.82 %) followed by those aged 4 to 8 years (39.13 %). It was least (8.69 %) in older dogs above 12 years and intermediate (4.30 %) in younger dogs less than 4 years of age. Breed wise higher occurrence was found in German Shepherd and Labrador Retriever as compared to other breeds. Cases of mammary tumours were observed only in females (100 %). There was an increasing frequency of canine mammary tumour from cranial thoracic to inguinal pairs with the highest incidence in caudal abdominal (41.17 %) and the least in caudal thoracic pair (2.94 %). Out of the 23 bitches, 95.65 per cent were intact and others were neutered. Radiography of thorax was performed to diagnose tumour metastasis in lung. It revealed absent of metastases in all the cases. Ultrasonography revealed size, irregularities of margins as well as echogenicity of mammary tumour masses with vasculature, helping to differentiate their nature and clinical significance. Haematobiochemical parameters did not show any remarkable variation in the cases of mammary tumours. Dendritic cells immunotherapy (Group 1) was performed in 8 cases. After one month of completion of therapy, size of tumour mass was increased gradually in four cases, in three cases growth remained constant in size and in one case it disappeared. Results of MTT assay and IFNG+ indicated that DC therapy potentiate CMI response in this group of animals. Surgical removal of mammary tumour (Group 2) was performed in 7 cases with good success and recurrence was noticed 2 months later in one case. Surgery followed by DC immunotherapy (Group 3) was performed in 8 cases. After completion of therapeutic protocol every case recovered uneventfully and no recurrence was observed. Data of MTT assay and IFNG+ indicated that DC therapy potentiate CMI response in this group of animals. Based on result, Surgery followed by DC therapy group was proved good compared to immunotherapy group and surgical group. Histopathology of selected 12 cases and FNAB of selected 6 cases of canine mammary tumours studied, 10 (55.55 %) cases were confirmed as benign mammary tumours like Cystic papillary adenoma, Benign mixed mammary tumour, Papillary fibroadenoma, Chondro fibro adenoma and Intra ductal papilloma, while 8 (44.44 %) were malignant mammary tumours like Papillary adenocarcinoma, Malignant myoepithelioma, Solid carcinoma and Malignant mix tumour. From the above study it can be concluded that DC therapy was observed to be safe and well tolerated in all animals without any adverse effects or toxicity. Study provided novel insights on the benefit of DC based cellular immunotherapy for managing mammary tumours in canines. Further studies to improve the therapeutic efficacy of DC therapy in canines are required in the future.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810039445
dc.keywordsMANAGEMENT, CANINE, MAMMARY TUMOURS, DENDRITIC CELL THERAPYen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.subVeterinary Surgeryen_US
dc.subjectveterinary science, surgery, radiologyen_US
dc.subjectstudyen_US
dc.titleSTUDIES ON MANAGEMENT OF CANINE MAMMARY TUMOURS WITH DENDRITIC CELL THERAPYen_US
dc.typeThesisen_US
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