DEVELOPMENT OF A NOVEL NANO CALCIUM FORTIFIED VALUE ADDED GOAT MEAT SPREAD
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Date
2022
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Abstract
A study was conducted to develop novel nano calcium incorporated value added goat meat spread. During development of spread preliminary trials were conducted to standardize the formulation with paprika (0.5, 1.0 & 1.5 %), honey (1.0, 3.0 & 5.0 %) butter (1.0, 3.0 & 5.0 %). Optimum level of calcium
carbonate (25, 50 & 75 %) and calcium citrate (25, 50 & 75 %) as per RDA prescribed by ICMR was assessed. Method for preparation of nano calcium carbonate and nano calcium citrate by ball milling was standardized and the yield and time required for synthesis of calcium was determined. The synthesized nano particles were characterized by assessing concentration of calcium, particle size, shape and zeta potential and were subjected to Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometer (XRD) and Energy Dispersive X-ray Spectroscopy (EDAX). In vitro cytotoxicity was assessed by MTT assay. After synthesis, characterization and cytotoxicity study conformation nano calcium particles were incorporated into the goat meat spread and optimum level of nano calcium carbonate (25, 50 & 75 %) and nano calcium citrate (25, 50 & 75 %) was assessed. The bioavailability and absorption of micro and nano calcium was determined by human gastrointestinal digestion simulation using a static in vitro method. Developed micro and nano calcium incorporated value added goat meat spread were packed and stored under refrigeration (4+1˚C) storage for further storage studies.