BIOLOGICAL AND MOLECULAR DETECTION OF APPLE STEM GROOVING VIRUS IN APPLE
Loading...
Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UHF,NAUNI,SOLAN
Abstract
ABSTRACT
Surveys conducted during 2009 and 2010 in 20 apple orchards of Shimla district
of Himachal Pradesh revealed viral disease incidence ranging from 5 to 95 per cent.
Chlorotic spots coupled with necrotic lesions on apple leaves were the predominant
symptoms. An orchard having 95 per cent incidence of viral diseases was selected for
conducting biological and serological detection of apple stem grooving virus (ASGV)
and other apple viruses. Serological detection through DAC and DAS-ELISA resulted in
the detection of apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus
(ASPV), apple mosaic virus (ApMV) with ASGV in the form of mixed infection in all
the samples of 10 cultivars randomly marked for assays. Biological detection of one of
the isolate containing infection of ASGV, ACLSV, ASPV and ApMV on herbaceous
hosts resulted in the production of symptoms on Chenopodium quinoa, Phaseolus
vulgaris, Nicotiana glutinosa and few other hosts. Detection on woody indicators
(Virginia crab, M. platycarpa, Spy 227, Jay Darling and Russian Clone) under field
conditions through double grafting, grafting cum budding and double budding of
inoculators and indicator budwood resulted in the production of typical viral symptoms
on leaves in M. platycarpa, Spy 227, Jay Darling and Russian Clone whereas swelling
and necrotic symptoms were produced at the graft union in case of Virginia crab and
Spy 227 indicators, respectively. Leaf samples drawn during March to May months
were found suitable for the ELISA of ASGV, ACLSV and ASPV whereas petals were
the best source for ApMV detection. Association of ASGV with viral symptoms in apple
was also confirmed by RT-PCR assay. Serological indexing resulted in the selection of
20 trees of 11 cultivars free from infection of ASGV, ACLSV, ASPV, ApMV and
tomato ringspot virus (ToRSV) in ELISA test of 60 symptomless trees of 20 cultivars.
Description
Keywords
economics, biological phenomena, resins, productivity, technological changes, exhibitions, developmental stages, marketing, biological development, selection