Process Development for Microwave Assisted Extraction of Glycyrrihizic acid from Mulhatti
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Date
2019
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CCSHAU
Abstract
In the present study, extraction of Glycyrrihizic acid from Mulhatti root powder was carried out using modified microwave system, rotary evaporator and HPLC unit. The effect of operational parameters i.e. microwave power (300, 325, 350, 375 and 400 W), extraction time (2, 3, 4, 5 and 6 min.) and by changing the different solvents for extraction (methanol and ethanol), powder size (0.80, 0.30, 0.15 mm) on extraction yield and GA were studied. Fixed ratio samples (1:10) i.e. mulhatti root powder, solvent was used during the experiment conducted. The amount of extracts of mulhatti roots was evaluated by using High Performance Liquid Chromatography (HPLC) with C18 column with a mobile phase consisting ethanol: water 70:30(v/v). Extraction of GA% using ethanol varied from 2.139% to 2.186% of mulhatti powder while from 0.285% to 0.307% using methanol as solvent. Further study was carried out using ethanol as solvent. Extraction yield of GA was 2.951% as maximum and minimum GA 0.2741% using different sizes (0.80, 0.30, 0.15 mm) of mulhatti powder. The optimized values of process parameters for maximum extraction yield% (24.54) and GA% (2.164) were microwave power 350 (W) and extraction time 4 min within the experimental region.
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